Smooth Muscle Actin antibody (N-Term)
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- Target See all Smooth Muscle Actin (ACTA2) Antibodies
- Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))
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Binding Specificity
- N-Term
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Smooth Muscle Actin antibody is un-conjugated
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Application
- Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificity
- Actin is a major component of the cytoskeleton and is present in most cell types. This MAb is highly specific to actin from smooth muscles. Its epitope lies in the first four N-terminal amino acids. This MAb does not stain cardiac or skeletal muscle, however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.
- Cross-Reactivity (Details)
- Human. Baboon. Monkey. Cow. Pig. Sheep. Goat. Cat. Dog. Rabbit. Mouse. Rat. Guinea Pig. Chicken.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- N-Terminal decapeptide of alpha smooth muscle isoform of actin and conjugated to KLH.
- Clone
- 1A4-same as asm-1
- Isotype
- IgG2a kappa
- Top Product
- Discover our top product ACTA2 Primary Antibody
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- Application Notes
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Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-4 μg/mL), ,Immunohistochemistry (Formalin-fixed) (0.25-0.5 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes), Optimal dilution for a specific application should be determined.
Positive_Control: HeLa cells (FACS/IF). Blood vessels in all tissues, smooth muscle or leiomyosarcoma (IHC).
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))
- Alternative Name
- ACTA2 (ACTA2 Products)
- Synonyms
- AAT6 antibody, ACTSA antibody, MYMY5 antibody, GAMMA-ACTIN antibody, ACT antibody, ACTA3 antibody, ACTE antibody, ACTL3 antibody, ACTSG antibody, Act-4 antibody, Act4 antibody, SMGA antibody, 0610041G09Rik antibody, Actvs antibody, SMalphaA antibody, a-SMA antibody, alphaSMA antibody, wu:fb63d03 antibody, ASMA antibody, SMactin antibody, a-sma antibody, aat6 antibody, actsa antibody, mymy5 antibody, sm-actin antibody, ACTA2 antibody, actc1 antibody, ACT-4 antibody, asma antibody, ACTGE antibody, actin, alpha 2, smooth muscle, aorta antibody, actin, gamma 2, smooth muscle, enteric antibody, actin, alpha 2, smooth muscle, aorta L homeolog antibody, ACTA2 antibody, ACTG2 antibody, Actg2 antibody, Acta2 antibody, acta2 antibody, acta2.L antibody
- Background
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ACTA2, Actin Alpha 2 Smooth Muscle Aorta, Actin Aortic Smooth Muscle, Actin Vascular Smooth Muscle, ACTSA, ACTVS, Alpha 2 Actin, Alpha Actin 2, Alpha Cardiac Actin, Alpha Smooth Muscle, Alpha-actin-2, Aortic Smooth Muscle, ASMA, Cell Growth-inhibiting Gene 46 Protein, Growth Inhibiting Gene 46,Actin, Smooth Muscle (Leiomyosarcoma Marker)
Cellular localisation: Cytoplasmic - Molecular Weight
- 42kDa
- Gene ID
- 59, 500483
- UniProt
- P62736
- Pathways
- Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development
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