VPS35 antibody

Catalog No. ABIN6932980

Product Details anti-VPS35 Antibody

Target: VPS35 (Vacuolar Protein Sorting 35 (VPS35))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This VPS35 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF), Immunocytochemistry (ICC)

Specificity: Ubiquitous. Highly expressed in brain, colon, heart, kidney, ovary, placenta, skeletal muscle, small intestine, testis, thymus.,Detects ~92 kDa.

Cross-Reactivity: Human, Mouse, Rat

Purification: Protein G Purified

Immunogen: Full length recombinant human VSP35

Clone: 10A8

Isotype: IgG2a

Application Details

Application Notes:

• WB (1:1000)
• ICC/IF (1:200)
• IP (1:200)
• optimal dilutions for assays should be determined by the user.

Comment:

A 1:1000 dilution of ABIN6932980 was sufficient for detection of VPS35 in 10 μg of SH-SY5Y by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 50 % glycerol, 0.09 % Sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

Target Details for VPS35

Target: VPS35 (Vacuolar Protein Sorting 35 (VPS35))

Alternative Name: VPS35 (VPS35 Products)

Synonyms: MEM3 antibody, PARK17 antibody, vps35 antibody, MGC81959 antibody, bZ34G2.3 antibody, si:rp71-34g2.3 antibody, wu:fa66e03 antibody, wu:fb95c08 antibody, zgc:136268 antibody, 23.m05999 antibody, Mem3 antibody, VPS35 antibody, DKFZp459E1510 antibody, CG5625 antibody, DVps35 antibody, Dmel\\CG5625 antibody, Dvps35 antibody, dvps35 antibody, AI647796 antibody, mem3 antibody, VPS35, retromer complex component antibody, VPS35 retromer complex component L homeolog antibody, VPS35 retromer complex component antibody, vacuolar protein sorting 35 homolog (S. cerevisiae) antibody, vacuolar protein sorting-associated protein 35 antibody, vacuolar protein sorting 35 antibody, Vacuolar protein sorting 35 antibody, hypothetical protein antibody, VPS35 retromer complex component S homeolog antibody, retromer complex subunit Vps35 antibody, VPS35 antibody, vps35.L antibody, vps35 antibody, Tc00.1047053511367.170 antibody, Tb10.70.5460 antibody, BBOV_IV009740 antibody, CC1G_12185 antibody, PTRG_08682 antibody, CMU_017400 antibody, PHATR_43830 antibody, MCYG_00751 antibody, MGYG_01212 antibody, EHI_086580 antibody, EHI_002990 antibody, EHI_041950 antibody, EHI_096520 antibody, Bm1_44045 antibody, LOC100285267 antibody, Vps35 antibody, PGTG_10018 antibody, Tsp_09825 antibody, vps35.S antibody

Background: Vacuolar Protein Sorter-35 (VPS35) is a component of the retromer complex, which is essential for endosome-to-Golgi retrieval of membrane proteins. VPS35 mutations such as D620N have been linked to Parkinson's Disease (PD) (1,2) and affect retromer function, protein homeostasis, and mitochondria (3).

Gene ID: 55737

NCBI Accession: NP_060676

UniProt: Q96QK1