CD63 antibody (AA 101-200)

Catalog No. ABIN687742

Product Details anti-CD63 Antibody

Target: CD63

Binding Specificity: AA 101-200

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD63 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Human, Mouse

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human CD63

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-CD63 antibody (ABIN687742)

Del Pozo-Acebo, Hazas, Tomé-Carneiro, Gil-Cabrerizo, San-Cristobal, Busto, García-Ruiz, Dávalos: "Bovine Milk-Derived Exosomes as a Drug Delivery Vehicle for miRNA-Based Therapy." in: International journal of molecular sciences, Vol. 22, Issue 3, (2021) (PubMed).

Marzesco, Flötenmeyer, Bühler, Obermüller, Staufenbiel, Jucker, Baumann: "Highly potent intracellular membrane-associated Aβ seeds." in: Scientific reports, Vol. 6, pp. 28125, (2016) (PubMed).

Sun, Zhao, Li, Wang, Nie, Peng, Wang, Zhang, Tian, Li, Song, Wang, Xu, Tian, Zhao, Xu, Zhong, Han, Ling, Chang, Li: "Osteoclast-derived microRNA-containing exosomes selectively inhibit osteoblast activity." in: Cell discovery, Vol. 2, pp. 16015, (2016) (PubMed).

Son, Rahimian, Shin, Siltanen, Patel, Revzin: "Microfluidic compartments with sensing microbeads for dynamic monitoring of cytokine and exosome release from single cells." in: The Analyst, (2015) (PubMed).

Target Details for CD63

Target: CD63

Alternative Name: CD63/MLA1 (CD63 Products)

Background:

Synonyms: MLA1, ME491, LAMP-3, OMA81H, TSPAN3, CD63 antigen, Granulophysin, Lysosomal-associated membrane protein 3, Melanoma-associated antigen ME491, Ocular melanoma-associated antigen, Tetraspanin-3, Tspan-3, CD63

Background: Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli.

Gene ID: 967

UniProt: P08962