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TLR3 antibody (AA 801-905)

Name: TLR3 antibody (AA 801-905)
SKU: ABIN686617
Price: 384.62 USD
Availability: InStock

TLR3 Reactivity: Human, Mouse, Rat WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

Formalin-fixed and paraffin embedded human placenta labeled with Rabbit Anti TLR3 Polyclonal Antibody, Unconjugated (ABIN686617) at 1:200 followed by conjugation to the secondary antibody and DAB staining (TLR3 antibody (AA 801-905))

IHC

Paraformaldehyde-fixed, paraffin embedded rat brain tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-TLR3 Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining (TLR3 antibody (AA 801-905))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded rat kidney tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-TLR3 Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining (TLR3 antibody (AA 801-905))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded rat lung tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-TLR3 Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining (TLR3 antibody (AA 801-905))

IHC (p)

4 images

(4 references)

Catalog No. ABIN686617

Datasheet as PDF

Target See all TLR3 Antibodies

TLR3 (Toll-Like Receptor 3 (TLR3))
Binding Specificity
15

10

8

8

5

4

3

3

3

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 801-905
Reactivity
95

61

29

6

2

1

1

1
Human, Mouse, Rat
Host
80

31

3

2
Rabbit
Compatible Secondaries
Clonality
83

33
Polyclonal
Conjugate
48

18

15

8

5

5

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This TLR3 antibody is un-conjugated
Application
77

40

34

33

21

14

13

12

12

12

7

4

4

3

2
Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity

Human, Mouse, Rat

Predicted Reactivity

Dog,Pig

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from mouse TLR3

Isotype

IgG

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Application Notes

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Zhao, Zhang, Wang, Jin, Zhou, Chen, Su: "Activation of Toll-like receptor 3 promotes pathological corneal neovascularization by enhancement of SDF-1-mediated endothelial progenitor cell recruitment." in: Experimental eye research, Vol. 178, pp. 177-185, (2019) (PubMed).

Zhu, Xu, Jiang, Wang, Geng, Wu, Hussain, Gao, Han, Li, Lan, Ning, Zhang, Holmdahl, Meng, Lu: "Pristane induces autophagy in macrophages, promoting a STAT1-IRF1-TLR3 pathway and arthritis." in: Clinical immunology (Orlando, Fla.), Vol. 175, pp. 56-68, (2016) (PubMed).

Wang, Lei, Zhang, Zhao, Fang, Lai, Han, Xiao, Wang: "Xenobiotic Pregnane X Receptor (PXR) Regulates Innate Immunity via Activation of NLRP3 Inflammasome in Vascular Endothelial Cells." in: The Journal of biological chemistry, Vol. 289, Issue 43, pp. 30075-81, (2014) (PubMed).

Zhu, Meng, Jiang, Xu, Wang, Han, Lu: "Overexpression of toll-like receptor 3 in spleen is associated with experimental arthritis in rats." in: Scandinavian journal of immunology, Vol. 76, Issue 3, pp. 263-70, (2012) (PubMed).
Target

TLR3 (Toll-Like Receptor 3 (TLR3))

Alternative Name

TLR3 (TLR3 Products)

Synonyms

CD283 antibody, IIAE2 antibody, AI957183 antibody, zgc:123006 antibody, toll like receptor 3 antibody, toll-like receptor 3 antibody, TLR3 antibody, Tlr3 antibody, tlr3 antibody

Background

Synonyms: AI957183, Toll-like receptor 3, CD283, Tlr3
Background: Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR3 is a nucleotide-sensing TLR which is activated by double-stranded RNA, a sign of viral infection. Acts via the adapter TRIF/TICAM1, leading to NF-kappa-B activation, IRF3 nuclear translocation, cytokine secretion and the inflammatory response (By similarity).

Gene ID

142980

UniProt

Q99MB1

Pathways

TLR Signaling, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades