AKAP12 antibody (AA 701-900)

Catalog No. ABIN685732

Product Details anti-AKAP12 Antibody

Target: AKAP12 (A Kinase (PRKA) Anchor Protein 12 (AKAP12))

Binding Specificity: AA 701-900

Reactivity: Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AKAP12 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human,Dog

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human of human AKAP12

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for AKAP12

Target: AKAP12 (A Kinase (PRKA) Anchor Protein 12 (AKAP12))

Alternative Name: AKAP12/Gravin (AKAP12 Products)

Synonyms: akap12 antibody, cb27 antibody, esr1 antibody, fb72g12 antibody, id:ibd1202 antibody, sb:cb27 antibody, si:ch73-192g21.1 antibody, wu:fb72g12 antibody, wu:fc21c03 antibody, AKAP12 antibody, AI317366 antibody, SSeCKS antibody, Srcs5 antibody, Tsga12 antibody, AKAP12A antibody, AKAP12B antibody, AKAP12G antibody, AKAP250 antibody, A kinase (PRKA) anchor protein 12b antibody, A-kinase anchoring protein 12 antibody, A kinase (PRKA) anchor protein (gravin) 12 antibody, akap12b antibody, AKAP12 antibody, Akap12 antibody

Background:

Synonyms: A kinase PRKA anchor protein gravin 12, A kinase Anchor Protein 12, A kinase anchor protein 250 kDa, AKAP 12, AKAP 250, AKAP250, DKFZp686M0430, DKFZp686O0331, Gercelin, Gravin, Kinase scaffold protein gravin, Myasthenia gravis autoantigen gravin, Srcs5, SSeCKS, Tsga12.

Background: Gravin (also known as A Kinase Anchor Protein 12, AKAP12) was cloned from a cDNA expression library screened with serum, from a myasthenia gravis patient. to Gravin were found in 22 out of 72 myasthemia gravis patients and absent in sera of normal individuals. This protein is a myristoylated high molecular weight cytoplasmic protein expressed in endothelial cells, cultured fibroblasts and osteosarcoma cells and highly expressed in the brain. Gravin can bind kinases like PKA and PKC by different domains of the protein and can localize these kinases to specific domains in the cell. Upon differentiation of NT2 cells to neurons (NT2 N), Gravin expression is up regulated and enriched at the inner peripheral cortex in close proximity to the plasma membrane. In differentiated cells, gravin is co-localized with PKCbII, PKCa and PKA. Decreased binding of PKCa to gravin was found after activation of PKC.

Gene ID: 9590

Pathways: cAMP Metabolic Process