HIST1H1E antibody (pThr18)

Catalog No. ABIN685183

Product Details anti-HIST1H1E Antibody

Target: HIST1H1E (Histone H1.4 (HIST1H1E))

Binding Specificity: pThr18

Reactivity: Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HIST1H1E antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This phosphorylation site is found in Rat H1C as well, but not Human or Mouse H1C. This phosphorylation site is homologous to Thr18 in Rat H1C/H1D and Mouse H1D. Due to the highly conserved nature of these histones, this antibody may react with these members when phosphorylated at this site.

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human Histone H1.4 around the phosphorylation site of Thr18

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for HIST1H1E

Target: HIST1H1E (Histone H1.4 (HIST1H1E))

Alternative Name: Histone H1.4 (HIST1H1E Products)

Synonyms: H14 antibody, H1d antibody, H1f4 antibody, Hist1h1c antibody, Hist1h1e antibody, H1.4 antibody, H1E antibody, H1F4 antibody, H1s-4 antibody, dJ221C16.5 antibody, H1-4 antibody, H1e antibody, H1var2 antibody, histone cluster 1, H1d antibody, histone cluster 1 H1 family member e antibody, histone cluster 1, H1e antibody, histone H1.4 antibody, Hist1h1d antibody, HIST1H1E antibody, Hist1h1e antibody, LOC488269 antibody

Background:

Synonyms: H1E, H1.4, H1F4, H1s-4, dJ221C16.5, Histone H1.4, Histone H1b, Histone H1s-4, HIST1H1E

Background: Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).

Gene ID: 3008

UniProt: P10412