FHIT antibody (pTyr114)
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- Target See all FHIT Antibodies
- FHIT (Fragile Histidine Triad (FHIT))
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Binding Specificity
- pTyr114
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This FHIT antibody is un-conjugated
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Application
- ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Cross-Reactivity
- Human
- Predicted Reactivity
- Mouse,Rat,Dog,Horse,Rabbit
- Purification
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic phosphopeptide derived from human FHIT around the phosphorylation site of Tyr114
- Isotype
- IgG
- Top Product
- Discover our top product FHIT Primary Antibody
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- Application Notes
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ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Expiry Date
- 12 months
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- Target
- FHIT (Fragile Histidine Triad (FHIT))
- Alternative Name
- FHIT (FHIT Products)
- Synonyms
- AP3Aase antibody, FRA3B antibody, FHIT antibody, zgc:73176 antibody, AW045638 antibody, Fra14A2 antibody, LOC100223655 antibody, fragile histidine triad antibody, fragile histidine triad gene antibody, fragile histidine triad protein antibody, fragile histidine triad L homeolog antibody, FHIT antibody, Fhit antibody, fhit antibody, PY07476 antibody, fhit.L antibody
- Background
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Synonyms: FRA3B, AP3Aase, Bis(5'-adenosyl)-triphosphatase, AP3A hydrolase, Diadenosine 5',5'''-P1,P3-triphosphate hydrolase, Dinucleosidetriphosphatase, Fragile histidine triad protein, FHIT
Background: Cleaves P(1)-P(3)-bis(5'-adenosyl) triphosphate (Ap3A) to yield AMP and ADP. Can also hydrolyze P(1)-P(4)-bis(5'-adenosyl) tetraphosphate (Ap4A), but has extremely low activity with ATP. Modulates transcriptional activation by CTNNB1 and thereby contributes to regulate the expression of genes essential for cell proliferation and survival, such as CCND1 and BIRC5. Plays a role in the induction of apoptosis via SRC and AKT1 signaling pathways. Inhibits MDM2-mediated proteasomal degradation of p53/TP53 and thereby plays a role in p53/TP53-mediated apoptosis. Induction of apoptosis depends on the ability of FHIT to bind P(1)-P(3)-bis(5'-adenosyl) triphosphate or related compounds, but does not require its catalytic activity, it may in part come from the mitochondrial form, which sensitizes the low-affinity Ca(2+) transporters, enhancing mitochondrial calcium uptake. Functions as tumor suppressor.
- Gene ID
- 2272
- UniProt
- P49789
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