FOXO3 antibody (pSer253)

Catalog No. ABIN684718

Product Details anti-FOXO3 Antibody

Target: FOXO3 (Forkhead Box O3 (FOXO3))

Binding Specificity: pSer253

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXO3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This phosphorylation site is homologous to Ser252 in Mouse and Rat.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Sheep,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human FoxO3a around the phosphorylation site of Ser253

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-FOXO3 antibody (ABIN684718)

Morales, Abrigo, Acuña, Santos, Bader, Brandan, Simon, Olguin, Cabrera, Cabello-Verrugio: "Angiotensin-(1-7) attenuates disuse skeletal muscle atrophy in mice via its receptor, Mas." in: Disease models & mechanisms, Vol. 9, Issue 4, pp. 441-9, (2016) (PubMed).

Target Details for FOXO3

Target: FOXO3 (Forkhead Box O3 (FOXO3))

Alternative Name: Foxo3a (FOXO3 Products)

Synonyms: AF6q21 antibody, FKHRL1 antibody, FKHRL1P2 antibody, FOXO2 antibody, FOXO3A antibody, zgc:92176 antibody, wu:fb50g02 antibody, wu:fi33f07 antibody, Fkhrl1 antibody, Foxo3a antibody, FoxO3A antibody, 1110048B16Rik antibody, 2010203A17Rik antibody, C76856 antibody, Fkhr2 antibody, FOXO-3 antibody, FOXO3 antibody, foxo3a antibody, xfoxo3 antibody, foxO3 antibody, foxO antibody, fkhr antibody, foxo5 antibody, wu:fj65a12 antibody, zFkhr antibody, forkhead box O3 antibody, forkhead box O3A antibody, forkhead box O3 L homeolog antibody, forkhead box O3b antibody, FOXO3 antibody, foxo3a antibody, Foxo3 antibody, foxo3.L antibody, foxo3 antibody, foxO3 antibody, foxo3b antibody

Background:

Synonyms: FOXO2, AF6q21, FKHRL1, FOXO3A, FKHRL1P2, Forkhead box protein O3, AF6q21 protein, Forkhead in rhabdomyosarcoma-like 1, FOXO3

Background: Transcriptional activator which triggers apoptosis in the absence of survival factors, including neuronal cell death upon oxidative stress. Recognizes and binds to the DNA sequence 5'-[AG]TAAA[TC]A-3'. Participates in post-transcriptional regulation of MYC: following phosphorylation by MAPKAPK5, promotes induction of miR-34b and miR-34C expression, 2 post-transcriptional regulators of MYC that bind to the 3'UTR of MYC transcript and prevent its translation.

Gene ID: 2309

UniProt: O43524

Pathways: Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis