EPH Receptor A2 antibody (pTyr594)

Catalog No. ABIN684508

Product Details anti-EPH Receptor A2 Antibody

Target: EPH Receptor A2 (EPHA2)

Binding Specificity: pTyr594

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPH Receptor A2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This phosphorylation site is homologous to Tyr595 in Mouse and Rat. Due to the highly conserved nature of this protein to EphA4 and EphA5, this antibody may react with these EphA4 when phosphorylated at Tyr602 (hu, mu, rt) and EphA5 when phosphorylated at Tyr656 in Human, Tyr495 in Mouse, and Tyr658 in Rat.

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Cow,Pig,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human EphA2 around the phosphorylation site of Tyr594

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for EPH Receptor A2

Target: EPH Receptor A2 (EPHA2)

Alternative Name: EphA2 (EPHA2 Products)

Synonyms: ARCC2 antibody, CTPA antibody, CTPP1 antibody, CTRCT6 antibody, ECK antibody, epha2 antibody, MGC89505 antibody, AW545284 antibody, Eck antibody, Myk2 antibody, Sek-2 antibody, Sek2 antibody, id:ibd5152 antibody, rtk6 antibody, G42 antibody, XE10 antibody, epha2-A antibody, EPH receptor A2 antibody, Eph receptor A2 antibody, epoxide hydrolase antibody, eph receptor A2 a antibody, EPH receptor A2 L homeolog antibody, EPHA2 antibody, Epha2 antibody, epha2 antibody, ephA2 antibody, epha2a antibody, epha2.L antibody

Background:

Synonyms: ECK, CTPA, ARCC2, CTPP1, CTRCT6, Ephrin type-A receptor 2, Epithelial cell kinase, Tyrosine-protein kinase receptor ECK, EPHA2

Background: Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Activated by the ligand ephrin-A1/EFNA1 regulates migration, integrin-mediated adhesion, proliferation and differentiation of cells. Regulates cell adhesion and differentiation through DSG1/desmoglein-1 and inhibition of the ERK1/ERK2 (MAPK3/MAPK1, respectively) signaling pathway. May also participate in UV radiation-induced apoptosis and have a ligand-independent stimulatory effect on chemotactic cell migration. During development, may function in distinctive aspects of pattern formation and subsequently in development of several fetal tissues. Involved for instance in angiogenesis, in early hindbrain development and epithelial proliferation and branching morphogenesis during mammary gland development. Engaged by the ligand ephrin-A5/EFNA5 may regulate lens fiber cells shape and interactions and be important for lens transparency development and maintenance. With ephrin-A2/EFNA2 may play a role in bone remodeling through regulation of osteoclastogenesis and osteoblastogenesis.

Gene ID: 1969

UniProt: P29317

Pathways: RTK Signaling