CDC25C antibody (pSer216)

Catalog No. ABIN684073

Product Details anti-CDC25C Antibody

Target: CDC25C (Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C))

Binding Specificity: pSer216

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC25C antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This phosphorylation site is found at Ser173 in Isoform 3 of this protein.

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human cdc25C around the phosphorylation site of Ser216

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for CDC25C

Target: CDC25C (Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C))

Alternative Name: cdc25C (CDC25C Products)

Synonyms: CDC25C antibody, cdc25 antibody, xcdc25c antibody, CDC25 antibody, PPP1R60 antibody, Cdc25 antibody, cdc25-3 antibody, xcdc25-3 antibody, cell division cycle 25C antibody, cell division cycle 25C S homeolog antibody, CDC25C antibody, cdc25c antibody, Cdc25c antibody, cdc25c.S antibody

Background:

Synonyms: CDC25, PPP1R60, M-phase inducer phosphatase 3, Dual specificity phosphatase Cdc25C, CDC25C

Background: Functions as a dosage-dependent inducer in mitotic control. Tyrosine protein phosphatase required for progression of the cell cycle. When phosphorylated, highly effective in activating G2 cells into prophase. Directly dephosphorylates CDK1 and activates its kinase activity.

Gene ID: 995

UniProt: P30307

Pathways: Cell Division Cycle, M Phase