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CD19 antibody (pTyr531)

Name: CD19 antibody (pTyr531)
SKU: ABIN683623
Price: 384.62 USD
Availability: InStock

CD19 Reactivity: Human, Mouse FACS, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) Host: Rabbit Polyclonal unconjugated

Formalin-fixed and human esophageal cancer tissue labeled with Anti-Phospho-CD19(Tyr531) Polyclonal Antibody, Unconjugated (ABIN683623) at 1:200 followed by conjugation to the secondary antibody and DAB staining (CD19 antibody (pTyr531))

IHC

Formalin-fixed and human esophageal cancer tissue labeled with Anti-Phospho-CD19(Tyr531) Polyclonal Antibody, Unconjugated at 1:200 followed by conjugation to the secondary antibody and DAB staining (CD19 antibody (pTyr531))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded mouse fetal liver tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with CD19 (Tyr531) Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining (CD19 antibody (pTyr531))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded mouse spleen tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with CD19 (Tyr531) Polyclonal Antibody, Unconjugated at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining (CD19 antibody (pTyr531))

IHC (p)

5 images

Catalog No. ABIN683623

Datasheet as PDF

Target See all CD19 Antibodies

CD19 (CD19 Molecule (CD19))
Binding Specificity
29

19

16

16

10

7

5

4

4

4

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pTyr531
Reactivity
286

157

48

31

19

10

7

5

4

3

2

2

2

2

2

2

2

2
Human, Mouse
Host
168

137

43

4
Rabbit
Compatible Secondaries
Clonality
220

128

2
Polyclonal
Conjugate
113

24

23

21

18

8

8

7

7

7

6

5

5

4

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1
This CD19 antibody is un-conjugated
Application
249

118

70

62

50

45

21

21

13

13

10

9

5

4

3

3

2

2

2

1

1

1

1

1

1

1
Flow Cytometry (FACS), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity

Human, Mouse

Predicted Reactivity

Rat,Dog,Cow,Pig,Rabbit,Guinea Pig

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic phosphopeptide derived from human CD19 around the phosphorylation site of Tyr531 [DS(p-Y)EN]

Isotype

IgG

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CD19 Reactivity: Human FACS, WB, IF, ICC, IHC (p) Host: Rabbit Polyclonal unconjugated

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CD19 Reactivity: Human FACS, WB, IHC, ELISA, IF Host: Rabbit Polyclonal unconjugated

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CD19 Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

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CD19 Reactivity: Human FACS, IP Host: Mouse Monoclonal LT19 unconjugated

anti-CD19 Molecule (CD19) antibody
CD19 Reactivity: Human FACS, ICC Host: Mouse Monoclonal 4G7 unconjugated

Application Notes

ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Target

CD19 (CD19 Molecule (CD19))

Alternative Name

CD19 (CD19 Products)

Synonyms

B4 antibody, CVID3 antibody, AW495831 antibody, CD19 antibody, CD19 molecule antibody, CD19 antigen antibody, CD19 antibody, Cd19 antibody

Background

Synonyms: B4, CVID3, B-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, Differentiation antigen CD19, T-cell surface antigen Leu-12, CD19
Background: Assembles with the antigen receptor of B-lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation.

Gene ID

930

UniProt

P15391

Pathways

Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway