B-Cell Linker antibody (pTyr96)

Catalog No. ABIN683458

Product Details anti-B-Cell Linker Antibody

Target: B-Cell Linker (BLNK)

Binding Specificity: pTyr96

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This B-Cell Linker antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human BLNK around the phosphorylation site of Tyr96 [DS(p-Y)EP]

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-B-Cell Linker antibody (ABIN683458)

Luger, Yang, Raviv, Weinberg, Banerjee, Lee, Trepel, Yang, Wakefield: "Expression of the B-cell receptor component CD79a on immature myeloid cells contributes to their tumor promoting effects." in: PLoS ONE, Vol. 8, Issue 10, pp. e76115, (2013) (PubMed).

Target Details for B-Cell Linker

Target: B-Cell Linker (BLNK)

Alternative Name: BLNK (BLNK Products)

Synonyms: BLNK antibody, blnk antibody, MGC147045 antibody, BASH antibody, Bca antibody, Ly-57 antibody, Ly57 antibody, Lyw-57 antibody, SLP-65 antibody, AGM4 antibody, BLNK-S antibody, LY57 antibody, SLP65 antibody, bca antibody, B-cell linker antibody, B cell linker antibody, BLNK antibody, blnk antibody, Blnk antibody

Background:

Synonyms: bca, AGM4, BASH, LY57, SLP65, BLNK-S, SLP-65, B-cell linker protein, B-cell adapter containing a SH2 domain protein, B-cell adapter containing a Src homology 2 domain protein, Cytoplasmic adapter protein, Src homology 2 domain-containing leukocyte protein of 65 kDa, BLNK

Background: Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. May play an important role in BCR-induced B-cell apoptosis.

Gene ID: 29760

UniProt: Q8WV28

Pathways: BCR Signaling