PRKAB1 antibody (pSer182)

Catalog No. ABIN683068

Product Details anti-PRKAB1 Antibody

Target: PRKAB1 (Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit (PRKAB1))

Binding Specificity: pSer182

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRKAB1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Flow Cytometry (FACS)

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human AMPK beta 1 around the phosphorylation site of Ser182

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for PRKAB1

Target: PRKAB1 (Protein Kinase, AMP-Activated, beta 1 Non-Catalytic Subunit (PRKAB1))

Alternative Name: Ampk beta 1 (PRKAB1 Products)

Synonyms: AMPK antibody, HAMPKb antibody, 1300015D22Rik antibody, AU021155 antibody, E430008F22 antibody, MGC82489 antibody, prkab1 antibody, wu:fk93d05 antibody, wu:fw87e09 antibody, zgc:56652 antibody, zgc:76975 antibody, zgc:92228 antibody, protein kinase AMP-activated non-catalytic subunit beta 1 antibody, protein kinase, AMP-activated, beta 1 non-catalytic subunit antibody, protein kinase, AMP-activated, beta 1 non-catalytic subunit S homeolog antibody, protein kinase, AMP-activated, beta 1 non-catalytic subunit, b antibody, protein kinase, AMP-activated, beta 1 non-catalytic subunit, a antibody, PRKAB1 antibody, Prkab1 antibody, prkab1.S antibody, prkab1 antibody, prkab1b antibody, prkab1a antibody

Background:

Synonyms: AMPK, HAMPKb, 5'-AMP-activated protein kinase subunit beta-1, AMPK subunit beta-1, AMPKb, PRKAB1

Background: Non-catalytic subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton, probably by indirectly activating myosin. Beta non-catalytic subunit acts as a scaffold on which the AMPK complex assembles, via its C-terminus that bridges alpha (PRKAA1 or PRKAA2) and gamma subunits (PRKAG1, PRKAG2 or PRKAG3).

Gene ID: 5564

UniProt: Q9Y478

Pathways: AMPK Signaling, Warburg Effect