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eIF4EBP1 antibody (pSer65)

EIF4EBP1 Reactivity: Human, Mouse, Rat WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN682963
  • Target See all eIF4EBP1 (EIF4EBP1) Antibodies
    eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))
    Binding Specificity
    • 25
    • 20
    • 20
    • 18
    • 16
    • 15
    • 10
    • 10
    • 7
    • 7
    • 6
    • 6
    • 5
    • 4
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer65
    Reactivity
    • 208
    • 154
    • 109
    • 21
    • 14
    • 7
    • 5
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 224
    • 13
    • 4
    Rabbit
    Clonality
    • 212
    • 31
    Polyclonal
    Conjugate
    • 103
    • 22
    • 17
    • 11
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 6
    • 5
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    This eIF4EBP1 antibody is un-conjugated
    Application
    • 197
    • 82
    • 76
    • 66
    • 65
    • 42
    • 23
    • 21
    • 15
    • 11
    • 9
    • 8
    • 5
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    Cross-Reactivity
    Human, Mouse, Rat
    Predicted Reactivity
    Dog,Cow,Pig,Horse,Chicken,Rabbit
    Purification
    Purified by Protein A.
    Immunogen
    KLH conjugated synthetic phosphopeptide derived from mouse 4EBP1 around the phosphorylation site of Ser65
    Isotype
    IgG
    Top Product
    Discover our top product EIF4EBP1 Primary Antibody
  • Application Notes
    WB 1:300-5000
    ELISA 1:500-1000
    IHC-P 1:200-400
    IHC-F 1:100-500
    IF(IHC-P) 1:50-200
    IF(IHC-F) 1:50-200
    IF(ICC) 1:50-200
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 μg/μL
    Buffer
    0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    Expiry Date
    12 months
  • Target
    eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))
    Alternative Name
    e4EBP1 (EIF4EBP1 Products)
    Background

    Synonyms: 4e-bp1, PHAS-I, AA959816, Eukaryotic translation initiation factor 4E-binding protein 1, eIF4E-binding protein 1, Phosphorylated heat- and acid-stable protein regulated by insulin 1, Eif4ebp1

    Background: Regulates eIF4E activity by preventing its assembly into the eIF4F complex: hypophosphorylated form competes with EIF4G1/EIF4G3 and strongly binds to EIF4E, leading to repress translation. Mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways.

    Gene ID
    13685
    UniProt
    Q60876
    Pathways
    MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Regulation of Cell Size, BCR Signaling
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