HES1 antibody (AA 41-150)

Catalog No. ABIN682393

Product Details anti-HES1 Antibody

Target: HES1 (Hes Family bHLH Transcription Factor 1 (HES1))

Binding Specificity: AA 41-150

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HES1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Cow,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human HES1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-HES1 antibody (ABIN682393)

Shi, Shu, Yang, Xu, Xing, Liu, Chen, Qi, Liu, Wang, Tang, Xie: "Wnt and Notch signaling pathway involved in wound healing by targeting c-Myc and Hes1 separately." in: Stem cell research & therapy, Vol. 6, pp. 120, (2015) (PubMed).

Gao, Zhang, Wang, Liu, Zheng, Yang, Huang, Ye, Luo, Xiao: "Hes1 is involved in the self-renewal and tumourigenicity of stem-like cancer cells in colon cancer." in: Scientific reports, Vol. 4, pp. 3963, (2014) (PubMed).

Long, Qiu, Liu, Fei, Feng, Wang, Zhong, Yi, Liu, Zhang, Han: "Functional recovery and neuronal regeneration of a rat model of epilepsy by transplantation of Hes1-down regulated bone marrow stromal cells." in: Neuroscience, Vol. 212, pp. 214-24, (2012) (PubMed).

Target Details for HES1

Target: HES1 (Hes Family bHLH Transcription Factor 1 (HES1))

Alternative Name: HES1 (HES1 Products)

Synonyms: HES-1 antibody, HHL antibody, HRY antibody, bHLHb39 antibody, Hry antibody, HAIRY1 antibody, c-hairy1A antibody, Xhairy1 antibody, hairy1 antibody, hes1 antibody, Xhairy-1 antibody, Xlh1 antibody, hes family bHLH transcription factor 1 antibody, hairy and enhancer of split 1 (Drosophila) antibody, hes family bHLH transcription factor 4 antibody, hes family bHLH transcription factor 1 S homeolog antibody, hes family bHLH transcription factor 1 L homeolog antibody, HES1 antibody, Hes1 antibody, HES4 antibody, hes1.S antibody, hes1.L antibody

Background:

Synonyms: HHL, HRY, HES-1, bHLHb39, Transcription factor HES-1, Class B basic helix-loop-helix protein 39, Hairy and enhancer of split 1, Hairy homolog, Hairy-like protein, HES1, HL

Background: Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Binds DNA on N-box motifs: 5'-CACNAG-3' with high affinity and on E-box motifs: 5'-CANNTG-3' with low affinity (By similarity). May play a role in a functional FA core complex response to DNA cross-link damage, being required for the stability and nuclear localization of FA core complex proteins, as well as for FANCD2 monoubiquitination in response to DNA damage.

Gene ID: 3280

UniProt: Q14469

Pathways: DNA Damage Repair