FZD5 antibody (PE-Cy7)

Catalog No. ABIN681866

Product Details anti-FZD5 Antibody (PE-Cy7)

Target: FZD5 (Frizzled Family Receptor 5 (FZD5))

Reactivity: Human, Mouse, Rat, Cow, Dog, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FZD5 antibody is conjugated to PE-Cy7

Application: Western Blotting (WB), Flow Cytometry (FACS)

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Pig,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Frizzled 5

Isotype: IgG

Application Details

Application Notes: IF (p) (1:100-500)
Not yet tested in other applications. Optimal working dilutions must be determined by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Protect from light.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for FZD5

Target: FZD5 (Frizzled Family Receptor 5 (FZD5))

Alternative Name: Frizzled 5 (FZD5 Products)

Background:

Synonyms: C2orf31, Frizzled homolog 5, Frizzled-5, Frizzled5, Fz 5, Fz-5, Fz5, FZD 5, hFz5, FZD5, FZD5_HUMAN, FzE 5, FzE5, HFZ 5, Seven transmembrane receptor frizzled 5, Wnt receptor, hFz8, Fz-8, Frizzled-8, FZD8.

Background: Members of the 'frizzled' gene family encode 7-transmembrane domain proteins that are receptors for Wnt signaling proteins. The Frizzled 5 protein is believed to be the receptor for the Wnt5A ligand. Frizzled 5 has been reported to be expressed in fetal kidney, fetal and adult liver, fetal lung, and adult pancreas. ESTs have been isolated from bone, liver/spleen, placenta, and prostate libraries. Frizzled 5 was cloned from a retina cDNA library. Receptor for Wnt proteins. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Coreceptor along with RYK of Wnt proteins, such as WNT1.

Molecular Weight: 62 kDa

Gene ID: 7855, 8325

Pathways: WNT Signaling