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Klotho antibody (AA 401-500)

Name: Klotho antibody (AA 401-500)
SKU: ABIN681778
Price: 384.62 USD
Availability: InStock

KL Reactivity: Human, Mouse, Rat IHC (p), IHC (fro), IF (p) Host: Rabbit Polyclonal unconjugated

Mouse kidney lysates probed with Klotho Polyclonal Antibody, unconjugated at 1:300 overnight at 4°C followed by a conjugated secondary antibody at 1:10000 for 90 minutes at 37°C. (Klotho antibody (AA 401-500))

Paraformaldehyde-fixed, paraffin embedded rat kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-Klotho Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by a conjugated secondary and DAB staining. (Klotho antibody (AA 401-500))

IHC (p)

Paraformaldehyde-fixed, paraffin embedded rat kidney tissue, Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min, Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes, Blocking buffer (normal goat serum) at 37°C for 20min, Antibody incubation with Rabbit Anti-klotho Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by a conjugated secondary and DAB staining (Klotho antibody (AA 401-500))

IHC (p)

Formalin-fixed and paraffin embedded mouse kidney labeled with Anti-Klotho Polyclonal Antibody, Unconjugated (ABIN681778) at 1:200 followed by conjugation to the secondary antibody and DAB staining (Klotho antibody (AA 401-500))

IHC

4 images

(1 reference)

Catalog No. ABIN681778

Datasheet as PDF

Target See all Klotho (KL) Antibodies

Klotho (KL)
Binding Specificity
4

3

3

3

3

3

3

2

1

1

1

1

1

1

1

1

1

1

1
AA 401-500
Reactivity
31

28

12

7

6

6

5

5

4

3

3

2

1
Human, Mouse, Rat
Host
41

4

2

1
Rabbit
Compatible Secondaries
Clonality
35

13
Polyclonal
Conjugate
29

7

4

1

1

1

1

1

1

1

1
This Klotho antibody is un-conjugated
Application
35

22

12

9

7

4

4

3

2

1

1
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity

Human, Mouse, Rat

Predicted Reactivity

Dog,Cow,Pig,Horse,Rabbit

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic peptide derived from human Klotho

Isotype

IgG

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KL Reactivity: Rat WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

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KL Reactivity: Human WB, FACS Host: Rabbit Polyclonal RB21226 unconjugated

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KL Reactivity: Human, Rat, Rabbit, Cow, Dog, Pig, Monkey IHC, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Klotho (KL) (AA 251-300) antibody
KL Reactivity: Human, Mouse, Rat, Rabbit, Horse, Dog, Guinea Pig, Bat, Monkey WB, IHC, IHC (p) Host: Rabbit Polyclonal unconjugated

anti-Klotho (KL) (Middle Region) antibody
KL Reactivity: Human, Mouse, Rat, Rabbit, Horse, Cow, Dog, Guinea Pig, Zebrafish (Danio rerio) WB, IHC Host: Rabbit Polyclonal unconjugated

anti-Klotho (KL) (Middle Region) antibody
KL Reactivity: Human, Mouse, Rat, Rabbit, Horse, Cow, Dog, Guinea Pig WB, IHC Host: Rabbit Polyclonal unconjugated

anti-Klotho (KL) (N-Term) antibody
KL Reactivity: Human WB Host: Rabbit Polyclonal unconjugated

anti-Klotho (KL) (Center) antibody
KL Reactivity: Human, Mouse, Rat WB, IHC (p), EIA, IF Host: Rabbit Polyclonal unconjugated

anti-Klotho (KL) (AA 765-981) antibody
KL Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

Application Notes

WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Prudhomme, Glinka, Kurt, Liu, Wang: "The anti-aging protein Klotho is induced by GABA therapy and exerts protective and stimulatory effects on pancreatic beta cells." in: Biochemical and biophysical research communications, Vol. 493, Issue 4, pp. 1542-1547, (2017) (PubMed).
Target

Klotho (KL)

Alternative Name

Klotho (KL Products)

Synonyms

alpha-kl antibody, KL antibody, LOC100223456 antibody, klotho antibody, Klotho antibody, KL antibody, Kl antibody, kl antibody, LOC100533542 antibody

Background

Synonyms: Klotho, KL
Background: May have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D (By similarity). Essential factor for the specific interaction between FGF23 and FGFR1 (By similarity). The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling.

Gene ID

9365

UniProt

Q9UEF7

Pathways

Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Hormone Activity, Growth Factor Binding