PARD3 antibody (AA 1201-1356)

Catalog No. ABIN681718

Product Details anti-PARD3 Antibody

Target: PARD3 (Par-3 Partitioning Defective 3 Homolog (PARD3))

Binding Specificity: AA 1201-1356

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PARD3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Cow,Pig,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PARD-3

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for PARD3

Target: PARD3 (Par-3 Partitioning Defective 3 Homolog (PARD3))

Alternative Name: PARD-3 (PARD3 Products)

Background:

Synonyms: Baz, ASIP, PAR3, PARD-3, PARD3A, SE2-5T2, PPP1R118, SE2-5L16, SE2-5LT1, PAR3alpha, Partitioning defective 3 homolog, PAR-3, Atypical PKC isotype-specific-interacting protein, CTCL tumor antigen se2-5, PAR3-alpha, PARD3, PAR3A

Background: Adapter protein involved in asymmetrical cell division and cell polarization processes. Seems to play a central role in the formation of epithelial tight junctions. Targets the phosphatase PTEN to cell junctions. Involved in Schwann cell peripheral myelination (By similarity). Association with PARD6B may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins. Required for establishment of neuronal polarity and normal axon formation in cultured hippocampal neurons.

Gene ID: 56288

UniProt: Q8TEW0

Pathways: Cell-Cell Junction Organization