FOXA2 antibody (AA 201-300)

Catalog No. ABIN679898

Product Details anti-FOXA2 Antibody

Target: FOXA2 (Forkhead Box A2 (FOXA2))

Binding Specificity: AA 201-300

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FOXA2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Cow,Pig,Horse,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human HNF 3 beta

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-FOXA2 antibody (ABIN679898)

Yamamizu, Schlessinger, Ko: "SOX9 accelerates ESC differentiation to three germ layer lineages by repressing SOX2 expression through P21 (WAF1/CIP1)." in: Development (Cambridge, England), Vol. 141, Issue 22, pp. 4254-66, (2014) (PubMed).

Yamamizu, Fujihara, Tachibana, Katayama, Takahashi, Hara, Imai, Shinkai, Yamashita: "Protein kinase A determines timing of early differentiation through epigenetic regulation with G9a." in: Cell stem cell, Vol. 10, Issue 6, pp. 759-70, (2012) (PubMed).

Target Details for FOXA2

Target: FOXA2 (Forkhead Box A2 (FOXA2))

Alternative Name: FOXA2/HNF 3beta (FOXA2 Products)

Synonyms: hnf3b antibody, tcf3b antibody, hnf3beta antibody, hnf3-beta antibody, FOXA2 antibody, HNF3B antibody, TCF3B antibody, HNF-3B antibody, ax antibody, axl antibody, fkd1 antibody, ik:tdsubc_2g8 antibody, tdsubc_2g8 antibody, wu:fe05g04 antibody, xx:tdsubc_2g8 antibody, FoxA2-A antibody, FoxA2a antibody, xFD-3 antibody, xbeta-1 antibody, HNF-3b antibody, HNF3-beta antibody, HNF3beta antibody, Hnf-3b antibody, Hnf3b antibody, Tcf-3b antibody, Tcf3b antibody, HNF-3BETA antibody, forkhead box A2 antibody, forkhead box A2 L homeolog antibody, foxa2 antibody, FOXA2 antibody, foxa2.L antibody, Foxa2 antibody

Background:

Synonyms: HNF3B, TCF3B, Hepatocyte nuclear factor 3-beta, HNF-3-beta, HNF-3B, Forkhead box protein A2, Transcription factor 3B, TCF-3B, FOXA2

Background: Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs, FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis, regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation.

Gene ID: 3170

UniProt: Q9Y261

Pathways: Dopaminergic Neurogenesis, Regulation of Carbohydrate Metabolic Process