EPO antibody (AA 101-193)

Catalog No. ABIN679718

Product Details anti-EPO Antibody

Target: EPO (Erythropoietin (EPO))

Binding Specificity: AA 101-193

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPO antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Dot Blot (DB)

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,Dog,Cow,Sheep,Pig,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Erythropoietin

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
dot-blot()

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-EPO antibody (ABIN679718)

Desharnais, Naud, Ayotte: "Detection of erythropoiesis stimulating agents in urine samples using a capillary Western system." in: Drug testing and analysis, Vol. 10, Issue 11-12, pp. 1698-1707, (2019) (PubMed).

Ichii, Nakamura, Irie, Kouguchi, Nakamura, Nakamura, Sato, Yokoyama, Horino, Sunden, Elewa, Kon: "Female cotton rats (Sigmodon hispidus) develop chronic anemia with renal inflammation and cystic changes." in: Histochemistry and cell biology, Vol. 146, Issue 3, pp. 351-62, (2016) (PubMed).

Li, Chen, Shao, Tang, Chen, Chen, Xu: "Oxidative stress induces the decline of brain EPO expression in aging rats." in: Experimental gerontology, Vol. 83, pp. 89-93, (2016) (PubMed).

DeNiro, Al-Mohanna: "Nuclear factor kappa-B signaling is integral to ocular neovascularization in ischemia-independent microenvironment." in: PLoS ONE, Vol. 9, Issue 7, pp. e101602, (2014) (PubMed).

Target Details for EPO

Target: EPO (Erythropoietin (EPO))

Alternative Name: EPO (EPO Products)

Synonyms: EPO antibody, EP antibody, MVCD2 antibody, erythropoietin antibody, erythropoietin S homeolog antibody, erythropoietin a antibody, EPO antibody, epo antibody, epo.S antibody, Epo antibody, epoa antibody

Target Type: Hormone

Background:

Synonyms: EP, MVCD2, Erythropoietin, EPO

Background: Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass.

Gene ID: 2056

UniProt: P01588

Pathways: JAK-STAT Signaling, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity