MTDH antibody (AA 131-230)

Catalog No. ABIN678863

Product Details anti-MTDH Antibody

Target: MTDH (Metadherin (MTDH))

Binding Specificity: AA 131-230

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MTDH antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Human

Predicted Reactivity: Mouse,Rat,Dog,Cow,Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human AEG-1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-MTDH antibody (ABIN678863)

Zhang, Li, Qian, Liu, Guo, Lin: "MTDH/AEG-1-based DNA vaccine suppresses metastasis and enhances chemosensitivity to paclitaxel in pelvic lymph node metastasis." in: Biomedicine & pharmacotherapy, Vol. 70, pp. 217-26, (2015) (PubMed).

Guo, Zhang, Liu, Wang, Liu, Wang, Ma, Wu: "Astrocyte elevated gene-1 overexpression in histologically favorable Wilms tumor is related to poor prognosis." in: Journal of pediatric urology, Vol. 10, Issue 2, pp. 317-23, (2014) (PubMed).

Zheng, Li, Wu, Liu, Sun, Yang, Hao, Sheng, Sun, Zhang, Long, Liang, Hu et al.: "Astrocyte elevated gene-1 (AEG-1) shRNA sensitizes Huaier polysaccharide (HP)-induced anti-metastatic potency via inactivating downstream P13K/Akt pathway as well as augmenting cell-mediated immune ..." in: Tumour biology, Vol. 35, Issue 5, pp. 4219-24, (2014) (PubMed).

Guo, Chen, Xi, Chang, Zhang, Zhang: "AEG-1 expression correlates with CD133 and PPP6c levels in human glioma tissues." in: Journal of biomedical research, Vol. 28, Issue 5, pp. 388-95, (2014) (PubMed).

Target Details for MTDH

Target: MTDH (Metadherin (MTDH))

Alternative Name: LYRIC (MTDH Products)

Synonyms: MGC75963 antibody, 2610103J23Rik antibody, 3D3 antibody, 3D3/Lyric antibody, AEG-1 antibody, AV353288 antibody, D8Bwg1112e antibody, Lyric antibody, AEG1 antibody, LYRIC antibody, LYRIC/3D3 antibody, metadherin antibody, mtdh antibody, MTDH antibody, Mtdh antibody

Background:

Synonyms: 3D3, AEG1, AEG-1, LYRIC, LYRIC/3D3, Protein LYRIC, 3D3/LYRIC, Astrocyte elevated gene-1 protein, Lysine-rich CEACAM1 co-isolated protein, Metadherin, Metastasis adhesion protein, MTDH

Background: Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance.

Gene ID: 92140

UniProt: Q86UE4

Pathways: Cellular Response to Molecule of Bacterial Origin, Cell-Cell Junction Organization