Ki-67 antibody (AA 1201-1300)

Catalog No. ABIN677858

Product Details anti-Ki-67 Antibody

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Binding Specificity: AA 1201-1300

Reactivity: Human, Mouse, Rat, Rabbit, Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ki-67 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Human, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human Ki67

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-Ki-67 antibody (ABIN677858)

Luo, Kong, Pei, Jin, Liu: "LncRNA CASC2 inhibits proliferation and migration of adenocarcinoma cells via miR-4735-3p and mTOR." in: Journal of cellular biochemistry, (2018) (PubMed).

Dong, Ma, Wang, Han, Zou, Wu, Zang, Zhuang: "YY1 promotes HDAC1 expression and decreases sensitivity of hepatocellular carcinoma cells to HDAC inhibitor." in: Oncotarget, Vol. 8, Issue 25, pp. 40583-40593, (2018) (PubMed).

Wu, Liu, Liu, Yan, Tang, Cui, Zhao, Shi, Hao, Yu, Qian: "A retrospective, single-center cohort study on 65 patients with primary retroperitoneal liposarcoma." in: Oncology letters, Vol. 15, Issue 2, pp. 1799-1810, (2018) (PubMed).

Thompson, Rahim, Arnold, Hielscher: "Loss of caveolin-1 alters extracellular matrix protein expression and ductal architecture in murine mammary glands." in: PLoS ONE, Vol. 12, Issue 2, pp. e0172067, (2017) (PubMed).

He, Chen, Yuan, Zhao, Chen, Wang, Li: "Tumor pH-Responsive Release of Drug-Conjugated Micelles from Fiber Fragments for Intratumoral Chemotherapy." in: ACS applied materials & interfaces, Vol. 9, Issue 38, pp. 32534-32544, (2017) (PubMed).

Liang, Fang, Yang, Song: "Silencing of CEMIP suppresses Wnt/β-catenin/Snail signaling transduction and inhibits EMT program of colorectal cancer cells." in: Acta histochemica, Vol. 120, Issue 1, pp. 56-63, (2017) (PubMed).

Antognelli, Gambelunghe, Muzi, Talesa et al.: "Glyoxalase I drives epithelial-to-mesenchymal transition via argpyrimidine-modified Hsp70, miR-21 and SMAD signalling in human bronchial cells BEAS-2B chronically exposed to crystalline silica ..." in: Free radical biology & medicine, Vol. 92, pp. 110-25, (2016) (PubMed).

Li, Zhu, Qiu: "Constructing aptamer anchored nanovesicles for enhanced tumor penetration and cellular uptake of water soluble chemotherapeutics." in: Acta biomaterialia, Vol. 35, pp. 269-79, (2016) (PubMed).

Drel, Tan, Barnes, Gorin, Lee, Wagner: "Centrality of bone marrow in the severity of gadolinium-based contrast-induced systemic fibrosis." in: FASEB journal : official publication of the Federation of American Societies for Experimental Biology, Vol. 30, Issue 9, pp. 3026-38, (2016) (PubMed).

Ma, Jia, Zhang: "IL-17 promotes keratinocyte proliferation via the downregulation of C/EBPα." in: Experimental and therapeutic medicine, Vol. 11, Issue 2, pp. 631-636, (2016) (PubMed).

Yang, Li, Zhu, Wang, Chen, Bai: "Hydroxysafflor yellow A inhibits angiogenesis of hepatocellular carcinoma via blocking ERK/MAPK and NF-?B signaling pathway in H22 tumor-bearing mice." in: European journal of pharmacology, Vol. 754, pp. 105-14, (2015) (PubMed).

Olianas, Dedoni, Onali et al.: "Antidepressants activate the lysophosphatidic acid receptor LPA(1) to induce insulin-like growth factor-I receptor transactivation, stimulation of ERK1/2 signaling and cell proliferation in CHO-K1 ..." in: Biochemical pharmacology, Vol. 95, Issue 4, pp. 311-23, (2015) (PubMed).

Zhang, Song, Pang, Zhang, Wan, Yuan, Wu, Liu: "Hydrogen-rich water protects against acetaminophen-induced hepatotoxicity in mice." in: World journal of gastroenterology : WJG, Vol. 21, Issue 14, pp. 4195-209, (2015) (PubMed).

Luo, Chen, Zhang, Chen, Li: "Pharmacokinetics and antitumor efficacy of micelles assembled from multiarmed amphiphilic copolymers with drug conjugates in comparison with drug-encapsulated micelles." in: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft für Pharmazeutische Verfahrenstechnik e.V, Vol. 98, pp. 9-19, (2015) (PubMed).

Kim, Lim, Kim, Kim, Kim, Tian, Park, Park, Choung: "The oncoprotein, gankyrin, is up-regulated in middle ear cholesteatoma." in: Acta oto-laryngologica, Vol. 134, Issue 3, pp. 238-43, (2014) (PubMed).

Ma, Yu, He, Xu, Wu, Xia, Guo, Wang, Ye, Zhang, Tao: "Involvement of c-KIT mutation in the development of gastrointestinal stromal tumors through proliferation promotion and apoptosis inhibition." in: OncoTargets and therapy, Vol. 7, pp. 637-43, (2014) (PubMed).

Ye, Zheng, Fan, Qin, Li, Tang, Ai, Zhang, Bian, Dang, Huang, Zhou, Zhou, Xiong, Yan, Ma, Li: "Lactoferrin deficiency promotes colitis-associated colorectal dysplasia in mice." in: PLoS ONE, Vol. 9, Issue 7, pp. e103298, (2014) (PubMed).

Li, Li, Yi, Luo, Guo, Pan, Gu: "Amphiphilic peptide dendritic copolymer-doxorubicin nanoscale conjugate self-assembled to enzyme-responsive anti-cancer agent." in: Biomaterials, Vol. 35, Issue 35, pp. 9529-45, (2014) (PubMed).

Luo, Zhang, Chen, Wei, Zhang, Li: "Antimetastasis and antitumor efficacy promoted by sequential release of vascular disrupting and chemotherapeutic agents from electrospun fibers." in: International journal of pharmaceutics, Vol. 475, Issue 1-2, pp. 438-449, (2014) (PubMed).

Pan, She, Guo, Luo, Yi, Gu: "PEGylated dendritic diaminocyclohexyl-platinum (II) conjugates as pH-responsive drug delivery vehicles with enhanced tumor accumulation and antitumor efficacy." in: Biomaterials, Vol. 35, Issue 38, pp. 10080-92, (2014) (PubMed).

Target Details for Ki-67

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Alternative Name: Ki-67 proliferation Marker (MKI67 Products)

Synonyms: MKI67 antibody, kia antibody, mki67 antibody, MGC132156 antibody, D630048A14Rik antibody, Ki-67 antibody, Ki67 antibody, KIA antibody, MIB-1 antibody, marker of proliferation Ki-67 antibody, marker of proliferation Ki-67 L homeolog antibody, marker of proliferation Ki-67 S homeolog antibody, antigen identified by monoclonal antibody Ki 67 antibody, antigen identified by monoclonal antibody Ki-67 antibody, nucleolar protein interacting with the FHA domain of MKI67 antibody, MKI67 antibody, mki67 antibody, mki67.L antibody, mki67.S antibody, Mki67 antibody, NIFK antibody

Background:

Synonyms: KIA, MIB-, MIB-1, PPP1R15, Antigen KI-67, MKI67

Background: Proliferation Marker Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.

Gene ID: 4288

UniProt: P46013

Pathways: Glycosaminoglycan Metabolic Process