E Tag antibody (HRP)

Catalog No. ABIN677762

Product Details anti-E Tag Antibody (HRP)

Target: E Tag

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E Tag antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA

Sequence: GAPVPYPDPL EPR

Cross-Reactivity: Tag

Cross-Reactivity (Details): Recombinant fusion protein, E tag

Purification: Purified by Protein A.

Immunogen: KLH conjugated E Tag synthetic peptide (GAPVPYPDPLEPR)

Isotype: IgG

Application Details

Application Notes: WB 1:100-1000
IHC-P 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

References for anti-E Tag antibody (HRP) (ABIN677762)

Wang, Mellon, Bowder, Quinn, Shea, Wood, Xiang: "Escherichia coli surface display of single-chain antibody VRC01 against HIV-1 infection." in: Virology, Vol. 475, pp. 179-86, (2014) (PubMed).

Target Details for E Tag

Target: E Tag

Abstract: E Tag Products

Target Type: Tag

Background:

Synonyms: GAPVPYPDPLEPR tag antibody, GAPVPYPDPLEPR epitope tag antibody, E epitope tag antibody.

Background: E Tag is a commonly used epitope tag engineered onto the N- or C- terminus of a protein of interest so that the tagged protein can be analyzed and visualized. Because of the small size of the epitope, it is unlikely to alter the activity of the cloned sequence.