NOS2 antibody (AA 14-100)

Catalog No. ABIN677228

Product Details anti-NOS2 Antibody

Target: NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))

Binding Specificity: AA 14-100

Reactivity: Human, Mouse, Rat, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOS2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Chicken, Human, Mouse, Rat

Predicted Reactivity: Dog,Pig,Rabbit,Guinea Pig

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from mouse NOS-2

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-NOS2 antibody (ABIN677228)

Liu, Li, Yang, Niu, Zhao, Zhao, Wang: "Valproic acid attenuates inflammation of optic nerve and apoptosis of retinal ganglion cells in a rat model of optic neuritis." in: Biomedicine & pharmacotherapy, Vol. 96, pp. 1363-1370, (2018) (PubMed).

Zhao, Jiang, Wang, Geng, Wang, Han, Wang: "The Roles of Reactive Oxygen Species and Nitric Oxide in Perfluorooctanoic Acid-Induced Developmental Cardiotoxicity and l-Carnitine Mediated Protection." in: International journal of molecular sciences, Vol. 18, Issue 6, (2017) (PubMed).

Sun, Yang, Zhang, Zhao: "Esculentoside A ameliorates cecal ligation and puncture-induced acute kidney injury in rats." in: Experimental animals, (2017) (PubMed).

Xiong, Wang, Chu, Chen, Guo: "Salvianolic Acid B Restored Impaired Barrier Function via Downregulation of MLCK by microRNA-1 in Rat Colitis Model." in: Frontiers in pharmacology, Vol. 7, pp. 134, (2016) (PubMed).

Bai, Zhang, Li, Liu, Lou, Ma, Luo, Ren: "Somatostatin prevents lipopolysaccharide?induced neurodegeneration in the rat substantia nigra by inhibiting the activation of microglia." in: Molecular medicine reports, Vol. 12, Issue 1, pp. 1002-8, (2015) (PubMed).

Sun, Zhang, Guan, Zhou, Sun: "Alpha-lipoic acid attenuates trinitrobenzene sulfonic acid-induced ulcerative colitis in mice." in: International journal of clinical and experimental medicine, Vol. 8, Issue 1, pp. 358-67, (2015) (PubMed).

Wang, Fang, Hu, Li, Hong: "Seawater immersion aggravates sciatic nerve injury in rats." in: Experimental and therapeutic medicine, Vol. 9, Issue 4, pp. 1153-1160, (2015) (PubMed).

Pei, Wang et al.: "Propofol attenuates LPS-induced tumor necrosis factor-?, interleukin-6 and nitric oxide expression in canine peripheral blood mononuclear cells possibly through down-regulation of nuclear factor ..." in: The Journal of veterinary medical science / the Japanese Society of Veterinary Science, (2014) (PubMed).

Karadeniz, Cavuso?lu, Turkmen, Uyan?kgil, Karadeniz, Akdemir, Tuglu, Ates, Erbas: "Experimental comparison of protective characteristics of enalapril and trimetazidine in diabetic nephropathy." in: Renal failure, Vol. 36, Issue 8, pp. 1283-90, (2014) (PubMed).

Target Details for NOS2

Target: NOS2 (Nitric Oxide Synthase 2, Inducible (NOS2))

Alternative Name: iNOS (NOS2 Products)

Synonyms: HEP-NOS antibody, INOS antibody, NOS antibody, NOS2A antibody, NOS-II antibody, Nos-2 antibody, Nos2a antibody, i-NOS antibody, iNOS antibody, iNos antibody, NOS2a antibody, NOS2 antibody, BmNOS2 antibody, Nsl antibody, nos2 antibody, nitric oxide synthase 2 antibody, nitric oxide synthase 2, inducible antibody, inducible nitric oxide synthase antibody, inducible nitric-oxide synthase antibody, nanos C2HC-type zinc finger 2 antibody, NOS2 antibody, Nos2 antibody, nos2 antibody, LOC396821 antibody, NANOS2 antibody

Background:

Synonyms: iNOS, Nos-2, Nos2a, i-NOS, NOS-II, Nitric oxide synthase, inducible, Inducible NO synthase, Inducible NOS, Macrophage NOS, MAC-NOS, NOS type II, Peptidyl-cysteine S-nitrosylase NOS2, Nos2, Inosl

Background: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such COX2.

Gene ID: 18126

UniProt: P29477

Pathways: Retinoic Acid Receptor Signaling Pathway, Cellular Response to Molecule of Bacterial Origin, Inositol Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process