LAMP1 antibody (AA 301-417)

Catalog No. ABIN676088

Product Details anti-LAMP1 Antibody

Target: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Binding Specificity: AA 301-417

Reactivity: Human, Mouse, Rat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LAMP1 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Cross-Reactivity: Human, Monkey, Mouse, Rat

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human LAMP-1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200
ICC 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-LAMP1 antibody (ABIN676088)

Bechor, Nachmias, Elia, Haim, Vatarescu, Leikin-Frenkel, Gericke, Tarnovscki, Las, Rudich: "Adipose tissue conditioned media support macrophage lipid-droplet biogenesis by interfering with autophagic flux." in: Biochimica et biophysica acta, Vol. 1862, Issue 9, pp. 1001-1012, (2017) (PubMed).

Pan, Kang, Wang, Liu, Oshima, Huang, Zhang, Yearsley, Yu, Wang: "Black Raspberries Enhance Natural Killer Cell Infiltration into the Colon and Suppress the Progression of Colorectal Cancer." in: Frontiers in immunology, Vol. 8, pp. 997, (2017) (PubMed).

Guo, Zhao, Qiu, Deis, Huang, Tang, Chen: "Niemann-Pick type C2 deficiency impairs autophagy-lysosomal activity, mitochondrial function, and TLR signaling in adipocytes." in: Journal of lipid research, Vol. 57, Issue 9, pp. 1644-58, (2016) (PubMed).

Jeong, Lee, Yang, Seong: "Combination of macrophage inflammatory protein 1 alpha with existing therapies to enhance the antitumor effects on murine hepatoma." in: Journal of radiation research, (2014) (PubMed).

Sun, Huang, Wang, Yu, Li, Li, Hu, Hao, Ishag, Mao: "Porcine reproductive and respiratory syndrome virus induces autophagy to promote virus replication." in: Autophagy, Vol. 8, Issue 10, pp. 1434-47, (2012) (PubMed).

Target Details for LAMP1

Target: LAMP1 (Lysosomal-Associated Membrane Protein 1 (LAMP1))

Alternative Name: LAMP-1 (LAMP1 Products)

Synonyms: CD107a antibody, LAMPA antibody, LGP120 antibody, AI196048 antibody, Lamp-1 antibody, LEP100 antibody, LAMP-1 antibody, LGP-A antibody, Lgpa antibody, Lysosome-associated membrane protein 1 antibody, cd107a antibody, lamp-1 antibody, lamp1 antibody, lampa antibody, lgp120 antibody, rexp76 antibody, lysosomal associated membrane protein 1 antibody, lysosomal-associated membrane protein 1 antibody, lysosomal-associated membrane protein 1 L homeolog antibody, LAMP1 antibody, Lamp1 antibody, lamp1.L antibody

Background:

Synonyms: LAMPA, CD17a, LGP12, Lysosome-associated membrane glycoprotein 1, LAMP-1, Lysosome-associated membrane protein 1, CD17 antigen-like family member A, LAMP1

Background: Lysosome associated membrane protein (LAMP1), also known as lgp120 or lgpA, is a type 1 integral membrane protein that is transported from trans Golgi networks to endosomes and then lysosomes. Upon cell activation, LAMP1 transfer to the plasma membrane is dependent on a carboxyl terminal tyrosine based motif (YXXI). Perturbation in the spacing between the tyrosine based motif relative to the membrane abolishes lysosome localization of LAMP1. This mutant protein then cycles between the plasma membrane and the endosome. Cell surface LAMP1 and LAMP2 have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore they are possibly involved in the adhesion of PBMCs to the site of inflammation.

Gene ID: 3916

UniProt: P11279

Pathways: Autophagy