Histone 3 antibody (H3K9me2)
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- Target See all Histone 3 (H3) Antibodies
- Histone 3 (H3)
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Binding Specificity
- H3K9me2
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Reactivity
- Human, Mouse, Rat, Monkey
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Host
- Rabbit
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Clonality
- Monoclonal
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Conjugate
- This Histone 3 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP)
- Specificity
- Recognizes human pan-methyl Histone H3 (Lys9). Species cross-reactivity: mouse, rat, monkey. Sequence Homology: pig, cerevisiae, chicken, bovine, melanogaster, xenopus, zebrafish, elegans.
- Immunogen
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Synthetic peptide corresponding to the amino terminus of histone H3 in which Lys9 is di-methylated (KLH-coupled).
Type of Immunogen: Synthetic peptide - KLH conjugated - Isotype
- IgG
- Top Product
- Discover our top product H3 Primary Antibody
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- Application Notes
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Approved: IF, IP (1:100), WB (1:1000)
Usage: Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence (IF-IC) and Immunoprecipitation (Chromatin). Western Blot: 1:1000. Incubate membrane with diluted antibody in 5 % w/v BSA, 1X TBS, 0.1 %. Tween-20 at 4 °C with gentle shaking, overnight. Immunoprecipitation: 1:100. Immunofluorescence (IF-IC) 1:50. Chromatin Immunoprecipitation (CHIP): 1:25. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- 10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol, 0.02 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least1 year at -20°C.
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- Target
- Histone 3 (H3)
- Alternative Name
- Histone H3 (H3 Products)
- Synonyms
- H-3 antibody, histocompatibility 3 antibody, H3 antibody
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