PNPO antibody (AA 36-85)

Catalog No. ABIN6756661

Product Details anti-PNPO Antibody

Target: PNPO (Pyridoxamine 5'-Phosphate Oxidase (PNPO))

Binding Specificity: AA 36-85

Reactivity: Human, Mouse, Horse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PNPO antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human PNPO

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa36-85 of human PNPO (Q9NVS9, NP_060599). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Panda, Horse (100%), Galago, Rat, Hamster, Dog, Rabbit, Guinea pig (92%), Elephant, Bovine (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for PNPO

Target: PNPO (Pyridoxamine 5'-Phosphate Oxidase (PNPO))

Alternative Name: PNPO (PNPO Products)

Background: Name/Gene ID: PNPO

Synonyms: PNPO, Pyridoxine 5-phosphate oxidase, Pyridoxine-5-phosphate oxidase, Pyridoxamine-phosphate oxidase, PDXPO, Pyridoxal 5-phosphate synthase

Gene ID: 55163

NCBI Accession: NP_060599