RHEB antibody (AA 107-156)

Catalog No. ABIN6755465

Product Details anti-RHEB Antibody

Target: RHEB (Ras Homolog Enriched in Brain (RHEB))

Binding Specificity: AA 107-156

Reactivity: Human, Mouse, Rat, Cow, Chicken, Dog, Guinea Pig, Horse, Rabbit, Sheep, Monkey, Bat, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RHEB antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human RHEB

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa107-156 of human RHEB (Q15382, NP_005605). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Sheep, Elephant, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard (100%), Xenopus, Stickleback, Zebrafish (92%), Beetle (85%), Paramecium (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for RHEB

Target: RHEB (Ras Homolog Enriched in Brain (RHEB))

Alternative Name: RHEB (RHEB Products)

Background: Name/Gene ID: RHEB
Family: Ras GTPase superfamily IPR001806

Synonyms: RHEB, RHEB2, GTP-binding protein Rheb, Ras homolog enriched in brain

Gene ID: 6009

NCBI Accession: NP_005605

Pathways: RTK Signaling