UCP1 antibody (AA 101-200)

Catalog No. ABIN675413

Product Details anti-UCP1 Antibody

Target: UCP1 (Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1))

Binding Specificity: AA 101-200

Reactivity: Human, Mouse, Rat, Goat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This UCP1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Goat, Human, Mouse, Rat

Predicted Reactivity: Dog,Cow,Sheep,Horse,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human UCP-1

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

References for anti-UCP1 antibody (ABIN675413)

Hou, Shi, Cao, Xu, Hu, Wang: "Pig has no uncoupling protein 1." in: Biochemical and biophysical research communications, Vol. 487, Issue 4, pp. 795-800, (2017) (PubMed).

Sun, Zeng, Cao, Song, Zhang, Liu: "Vibration Training Triggers Brown Adipocyte Relative Protein Expression in Rat White Adipose Tissue." in: BioMed research international, Vol. 2015, pp. 919401, (2015) (PubMed).

Lu, Ji, Zhang, Zhang, Zhang, An, Liu, Zheng: "Resistance to obesity by repression of VEGF gene expression through induction of brown-like adipocyte differentiation." in: Endocrinology, Vol. 153, Issue 7, pp. 3123-32, (2012) (PubMed).

Target Details for UCP1

Target: UCP1 (Uncoupling Protein 1 (Mitochondrial, Proton Carrier) (UCP1))

Alternative Name: UCP-1 (UCP1 Products)

Synonyms: ARABIDOPSIS THALIANA PLANT UNCOUPLING MITOCHONDRIAL PROTEIN 1 antibody, ARABIDOPSIS THALIANA UNCOUPLING PROTEIN 1 antibody, ATPUMP1 antibody, ATUCP1 antibody, UCP antibody, UCP1 antibody, UNCOUPLING PROTEIN 1 antibody, plant uncoupling mitochondrial protein 1 antibody, ucp antibody, slc25a7 antibody, SLC25A7 antibody, AI385626 antibody, Slc25a7 antibody, Ucp antibody, Ucpa antibody, Uncp antibody, plant uncoupling mitochondrial protein 1 antibody, uncoupling protein 1 antibody, uncoupling protein 1 L homeolog antibody, uncoupling protein 1 (mitochondrial, proton carrier) antibody, PUMP1 antibody, UCP1 antibody, ucp1.L antibody, ucp1 antibody, Ucp1 antibody

Background:

Synonyms: Ucp, Slc25a7, AI385626, Mitochondrial brown fat uncoupling protein 1, UCP 1, Solute carrier family 25 member 7, Thermogenin, Ucp1

Background: UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat.

Gene ID: 22227

UniProt: P12242

Pathways: Proton Transport, Brown Fat Cell Differentiation