SMPD1 antibody (AA 396-445)

Catalog No. ABIN6752980

Product Details anti-SMPD1 Antibody

Target: SMPD1 (Sphingomyelin phosphodiesterase 1, Acid Lysosomal (SMPD1))

Binding Specificity: AA 396-445

Reactivity: Human, Mouse, Rat, Cow, Sheep, Dog, Horse, Rabbit, Pig, Bat, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMPD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human SMPD1

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa396-445 of human SMPD1 (P17405, NP_000534). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Sheep, Dog, Bovine, Bat, Rabbit, Horse, Pig (100%), Guinea pig (92%), Mouse, Rat, Elephant (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for SMPD1

Target: SMPD1 (Sphingomyelin phosphodiesterase 1, Acid Lysosomal (SMPD1))

Alternative Name: SMPD1 / Acid Sphingomyelinase (SMPD1 Products)

Synonyms: ASM antibody, ASMASE antibody, NPD antibody, A-SMase antibody, Zn-SMase antibody, aSMase antibody, SMPD1 antibody, sphingomyelin phosphodiesterase 1 antibody, sphingomyelin phosphodiesterase 1, acid lysosomal antibody, sphingomyelin phosphodiesterase antibody, SMPD1 antibody, Smpd1 antibody, LOC5578088 antibody

Background: Name/Gene ID: SMPD1
Family: Phosphodiesterase - Other

Synonyms: SMPD1, Acid sphingomyelinase, ASM, NPD, ASMASE

Gene ID: 6609

NCBI Accession: NP_000534