RHOT1 antibody (N-Term)

Catalog No. ABIN6749728

Product Details anti-RHOT1 Antibody

Target: RHOT1 (Ras Homolog Gene Family, Member T1 (RHOT1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat, Rabbit, Cow, Guinea Pig, Horse, Bat, Chicken, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RHOT1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human RHOT1

Predicted Reactivity: Percent identity by BLAST analysis: Rat, Rabbit, Horse, Pig, Guinea pig, Chicken (100%) Xenopus (92%) Dog (82%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from N-Terminus of human RHOT1 (Q8IXI2, NP_060777). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Seabass, Stickleback (100%), Panda, Dog, Xenopus, Zebrafish, Drosophila (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 - 10 μg/mL), WB

Usage: ELISA titer using peptide based assay: 1:62500. Western Blot: Suggested dilution at 0.25 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for RHOT1

Target: RHOT1 (Ras Homolog Gene Family, Member T1 (RHOT1))

Alternative Name: RHOT1 (RHOT1 Products)

Synonyms: rhot1 antibody, zgc:55581 antibody, zgc:77063 antibody, wu:fd14e11 antibody, wu:fi14a05 antibody, arht2 antibody, miro-2 antibody, rasl antibody, ARHT1 antibody, MIRO-1 antibody, MIRO1 antibody, 2210403N23Rik antibody, AA415293 antibody, AF244542 antibody, AI834919 antibody, Arht1 antibody, C430039G08Rik antibody, Miro1 antibody, miro1 antibody, si:dkeyp-97g3.6 antibody, ras homolog family member T1a antibody, ras homolog family member T2 antibody, ras homolog family member T1 antibody, ras homolog family member T1 L homeolog antibody, rhot1a antibody, rhot2 antibody, RHOT1 antibody, rhot1 antibody, rhot1.L antibody, Rhot1 antibody, rhot1b antibody

Background: Name/Gene ID: RHOT1
Family: Ras GTPase superfamily IPR001806

Synonyms: RHOT1, ARHT1, HMiro-1, MIRO-1, Mitochondrial Rho GTPase 1, MIRO1, Mitochondrial Rho 1, Ras homolog family member T1

Gene ID: 55288

NCBI Accession: NP_060777

UniProt: Q8IXI2