EIF4E antibody (C-Term)

Catalog No. ABIN6748583

Product Details anti-EIF4E Antibody

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Binding Specificity: C-Term

Reactivity: Human, Rat, Mouse, Zebrafish (Danio rerio), Cow, Rabbit, Monkey, Dog, Guinea Pig, Horse, Chicken, Pig, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EIF4E antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human EIF4E

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Pig, Guinea pig, Chicken, Xenopus, Zebrafish (100%) Bovine, Rabbit (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human EIF4E (P06730, NP_001959). Percent identity by BLAST analysis: Human, Chimpanzee, Gibbon, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Armadillo, Xenopus, Frog, Smelt, Zebrafish (100%), Bat, Trout, Salmon (92%), Gorilla, Monkey (85%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: ELISA titer using peptide based assay: 1:312500. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1:50000 - 100000 as second antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled Water.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for EIF4E

Target: EIF4E (Eukaryotic Translation Initiation Factor 4E (EIF4E))

Alternative Name: EIF4E (EIF4E Products)

Background: Name/Gene ID: EIF4E

Synonyms: EIF4E, EIF-4E, EIF4E1, EIF4EL1, EIF4F, CBP, EIF-4F 25 kDa subunit, MRNA cap-binding protein

Gene ID: 1977

NCBI Accession: NP_001959

UniProt: P06730

Pathways: BCR Signaling