BRD3 antibody (AA 648-697)

Catalog No. ABIN6748497

Product Details anti-BRD3 Antibody

Target: BRD3 (Bromodomain Containing 3 (BRD3))

Binding Specificity: AA 648-697

Reactivity: Human, Mouse, Rat, Dog, Horse, Rabbit, Zebrafish (Danio rerio), Guinea Pig, Cow, Chicken, Hamster, Monkey, Bat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BRD3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human BRD3

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Chicken (100%) Xenopus (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa648-697 of human BRD3 (Q15059, NP_031397). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus (100%), Xenopus (92%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (10 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute with 50 μL sterile ddH2O.

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for BRD3

Target: BRD3 (Bromodomain Containing 3 (BRD3))

Alternative Name: BRD3 (BRD3 Products)

Synonyms: ORFX antibody, RING3L antibody, 2410084F24Rik antibody, AW060456 antibody, Fsrg2 antibody, RINGL3 antibody, brd3 antibody, fi36b10 antibody, wu:fa13h06 antibody, wu:fc01d09 antibody, wu:fc74a08 antibody, wu:fi04h10 antibody, wu:fi36b10 antibody, BRD3 antibody, ring3l antibody, fa28f05 antibody, wu:fa28f05 antibody, wu:fc56a11 antibody, wu:fj66d12 antibody, zgc:77289 antibody, bromodomain containing 3 antibody, bromodomain containing 3a antibody, bromodomain containing 3 S homeolog antibody, bromodomain-containing protein 3 antibody, bromodomain containing 3b antibody, BRD3 antibody, Brd3 antibody, brd3a antibody, brd3.S antibody, LOC100410652 antibody, LOC100354641 antibody, brd3b antibody

Background: Name/Gene ID: BRD3
Subfamily: BRD
Family: Protein Kinase

Synonyms: BRD3, Bromodomain-containing 3, Fsrg2, KIAA0043, ORFX, RING3-like protein, RING3L, Bromodomain containing 3

Gene ID: 8019

NCBI Accession: NP_031397

UniProt: Q15059

Pathways: Chromatin Binding