TLE4 antibody (AA 215-264)

Catalog No. ABIN6748487

Product Details anti-TLE4 Antibody

Target: TLE4 (Transducin-like Enhancer Protein 4 (TLE4))

Binding Specificity: AA 215-264

Reactivity: Human, Rat, Mouse, Chicken, Dog, Guinea Pig, Horse, Rabbit, Bat, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TLE4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human TLE4

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit, Horse, Pig, Guinea pig, Chicken (100%) Bovine, Xenopus (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa215-264 of human TLE4 (Q04727, NP_008936). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Rabbit, Horse, Pig, Guinea pig, Zebra finch, Chicken (100%), Bovine, Platypus, Xenopus (92%), Opossum, Lizard (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for TLE4

Target: TLE4 (Transducin-like Enhancer Protein 4 (TLE4))

Alternative Name: GRG4 / TLE4 (TLE4 Products)

Synonyms: TLE4 antibody, NV17443 antibody, BCE-1 antibody, BCE1 antibody, E(spI) antibody, ESG antibody, ESG4 antibody, GRG4 antibody, Esp2 antibody, Grg4 antibody, XGrg-4 antibody, XTCF-4 antibody, Xgrg4 antibody, Xtle4 antibody, esg4 antibody, grg4 antibody, gro antibody, 5730411M05Rik antibody, AA792082 antibody, Bce-1 antibody, Bce1 antibody, ESTM13 antibody, ESTM14 antibody, X83332 antibody, X83333 antibody, transducin like enhancer of split 4 antibody, transducin-like enhancer protein 4 antibody, protein groucho-1 antibody, transducin-like enhancer of split 4 antibody, transducin like enhancer of split 4-Z 1 antibody, transducin like enhancer of split 4 S homeolog antibody, TLE4 antibody, LOC706619 antibody, LOC100117159 antibody, LOC100462498 antibody, Tle4 antibody, TLE4Z1 antibody, LOC476317 antibody, tle4.S antibody

Background: Name/Gene ID: TLE4

Synonyms: TLE4, BCE1, BCE-1, E(spI), Enhancer of split groucho 4, ESG, ESG4, GRG4, Protein BCE-1, KIAA1261, R-esp2, B lymphocyte gene 1

Gene ID: 7091

NCBI Accession: NP_008936

UniProt: Q04727

Pathways: WNT Signaling