CHFR antibody (AA 36-85)

Catalog No. ABIN6747082

Product Details anti-CHFR Antibody

Target: CHFR (Checkpoint with Forkhead and Ring Finger Domains (CHFR))

Binding Specificity: AA 36-85

Reactivity: Human, Mouse, Rat, Rabbit, Zebrafish (Danio rerio), Guinea Pig, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CHFR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Brand: IHC-plus™

Specificity: Human CHFR

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Rabbit, Guinea pig (100%) Dog, Bovine, Horse, Pig, Chicken (92%) Xenopus, Zebrafish (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa36-85 of human CHFR (Q96EP1, NP_060693). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Galago, Marmoset, Mouse, Rat, Rabbit, Guinea pig (100%), Elephant, Dog, Bovine, Bat, Horse, Pig, Opossum, Turkey, Zebra finch, Chicken, Platypus (92%), Xenopus, Salmon, Zebrafish (85%), Stickleback (84%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: IHC, IHC-P (2.5 μg/mL), WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:312500.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

Target Details for CHFR

Target: CHFR (Checkpoint with Forkhead and Ring Finger Domains (CHFR))

Alternative Name: CHFR (CHFR Products)

Background: Name/Gene ID: CHFR

Synonyms: CHFR, RING finger protein 196, RNF196, RNF116

Gene ID: 55743

NCBI Accession: NP_060693