CEACAM3 antibody (AA 177-226)

Catalog No. ABIN6746759

Product Details anti-CEACAM3 Antibody

Target: CEACAM3 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 3 (CEACAM3))

Binding Specificity: AA 177-226

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CEACAM3 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human CEACAM3

Predicted Reactivity: Percent identity by BLAST analysis:

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa177-226 of human CEACAM3 (P40198, NP_001806). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-CEACAM3 antibody (ABIN6746759)

Li, Trudel, Wogan: "Nitric oxide-induced genotoxicity, mitochondrial damage, and apoptosis in human lymphoblastoid cells expressing wild-type and mutant p53." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 16, pp. 10364-9, (2002) (PubMed).

Cain, Brown, Langlais, Cohen: "Caspase activation involves the formation of the aposome, a large (approximately 700 kDa) caspase-activating complex." in: The Journal of biological chemistry, Vol. 274, Issue 32, pp. 22686-92, (1999) (PubMed).

Hu, Benedict, Ding, Núñez: "Role of cytochrome c and dATP/ATP hydrolysis in Apaf-1-mediated caspase-9 activation and apoptosis." in: The EMBO journal, Vol. 18, Issue 13, pp. 3586-95, (1999) (PubMed).

Soengas, Alarcón, Yoshida, Giaccia, Hakem, Mak, Lowe: "Apaf-1 and caspase-9 in p53-dependent apoptosis and tumor inhibition." in: Science (New York, N.Y.), Vol. 284, Issue 5411, pp. 156-9, (1999) (PubMed).

Target Details for CEACAM3

Target: CEACAM3 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 3 (CEACAM3))

Alternative Name: CD66d / CEACAM3 (CEACAM3 Products)

Background: Name/Gene ID: CEACAM3
Family: CEA

Synonyms: CEACAM3, Carcinoembryonic antigen CGM1, CGM1, CD66d antigen, CEA, W264, W282, CD66D

Gene ID: 1084

NCBI Accession: NP_001806

UniProt: P40198