CEACAM3 antibody (AA 177-226)

Catalog No. ABIN6746759

Product Details anti-CEACAM3 Antibody

Target: CEACAM3 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 3 (CEACAM3))

Binding Specificity: AA 177-226

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CEACAM3 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human CEACAM3

Predicted Reactivity: Percent identity by BLAST analysis:

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa177-226 of human CEACAM3 (P40198, NP_001806). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-CEACAM3 antibody (ABIN6746759)

Li, Trudel, Wogan: "Nitric oxide-induced genotoxicity, mitochondrial damage, and apoptosis in human lymphoblastoid cells expressing wild-type and mutant p53." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 16, pp. 10364-9, (2002) (PubMed).

Cain, Brown, Langlais, Cohen: "Caspase activation involves the formation of the aposome, a large (approximately 700 kDa) caspase-activating complex." in: The Journal of biological chemistry, Vol. 274, Issue 32, pp. 22686-92, (1999) (PubMed).

Hu, Benedict, Ding, Núñez: "Role of cytochrome c and dATP/ATP hydrolysis in Apaf-1-mediated caspase-9 activation and apoptosis." in: The EMBO journal, Vol. 18, Issue 13, pp. 3586-95, (1999) (PubMed).

Soengas, Alarcón, Yoshida, Giaccia, Hakem, Mak, Lowe: "Apaf-1 and caspase-9 in p53-dependent apoptosis and tumor inhibition." in: Science (New York, N.Y.), Vol. 284, Issue 5411, pp. 156-9, (1999) (PubMed).

Target Details for CEACAM3

Target: CEACAM3 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 3 (CEACAM3))

Alternative Name: CD66d / CEACAM3 (CEACAM3 Products)

Synonyms: CD66D antibody, CEA antibody, CGM1 antibody, W264 antibody, W282 antibody, CGM4AA antibody, Cea antibody, Ceacam5 antibody, Cear antibody, Cgm1 antibody, Cgm4 antibody, RATCEAA antibody, RATCGM4AA antibody, EG384557 antibody, Psg24 antibody, cea12 antibody, carcinoembryonic antigen related cell adhesion molecule 3 antibody, carcinoembryonic antigen-related cell adhesion molecule 3 antibody, CEACAM3 antibody, Ceacam3 antibody

Background: Name/Gene ID: CEACAM3
Family: CEA

Synonyms: CEACAM3, Carcinoembryonic antigen CGM1, CGM1, CD66d antigen, CEA, W264, W282, CD66D

Gene ID: 1084

NCBI Accession: NP_001806

UniProt: P40198