DNAJC19 antibody (C-Term)

Catalog No. ABIN6746682

Product Details anti-DNAJC19 Antibody

Target: DNAJC19 (DnaJ (Hsp40) Homolog, Subfamily C, Member 19 (DNAJC19))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rabbit, Cow, Horse, Monkey, Pig, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DNAJC19 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human DNAJC19

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rabbit, Horse, Pig, Xenopus (100%) Chicken, Zebrafish (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of human DNAJC19 (Q96DA6, NP_660304). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey, Marmoset, Mouse, Elephant, Bovine, Rabbit, Horse, Pig, Platypus, Lizard, Xenopus (100%), Guinea pig, Zebra finch, Chicken, Zebrafish (92%), Opossum, Frog, Trout, Salmon (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-DNAJC19 antibody (ABIN6746682)

Straub, Grue, Uhse, Lisby, Knudsen, Tange, Westergaard, Boege: "The RNA-splicing factor PSF/p54 controls DNA-topoisomerase I activity by a direct interaction." in: The Journal of biological chemistry, Vol. 273, Issue 41, pp. 26261-4, (1998) (PubMed).

Basu, Dong, Krainer, Howe: "The intracisternal A-particle proximal enhancer-binding protein activates transcription and is identical to the RNA- and DNA-binding protein p54nrb/NonO." in: Molecular and cellular biology, Vol. 17, Issue 2, pp. 677-86, (1997) (PubMed).

Dong, Horowitz, Kobayashi, Krainer: "Purification and cDNA cloning of HeLa cell p54nrb, a nuclear protein with two RNA recognition motifs and extensive homology to human splicing factor PSF and Drosophila NONA/BJ6." in: Nucleic acids research, Vol. 21, Issue 17, pp. 4085-92, (1993) (PubMed).

Target Details for DNAJC19

Target: DNAJC19 (DnaJ (Hsp40) Homolog, Subfamily C, Member 19 (DNAJC19))

Alternative Name: DNAJC19 (DNAJC19 Products)

Background: Name/Gene ID: DNAJC19

Synonyms: DNAJC19, Homolog of yeast TIM14, PAM18, TIM14, TIMM14

Gene ID: 131118

NCBI Accession: NP_660304

UniProt: Q96DA6

Pathways: SARS-CoV-2 Protein Interactome