GUCA1B antibody (AA 34-83)

Catalog No. ABIN6746667

Product Details anti-GUCA1B Antibody

Target: GUCA1B (Guanylate Cyclase Activator 1B (Retina) (GUCA1B))

Binding Specificity: AA 34-83

Reactivity: Human, Mouse, Rat, Cow, Dog, Rabbit

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GUCA1B antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human GUCA1B / GCAP2

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Mouse (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa34-83 of human GUCA1B (Q9UMX6, NP_002089). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey (100%), Marmoset (92%), Mouse, Elephant (85%), Rat (78%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-GUCA1B antibody (ABIN6746667)

Giger, Urquhart, Gillespie, Levengood, Ginty, Kolodkin: "Neuropilin-2 is a receptor for semaphorin IV: insight into the structural basis of receptor function and specificity." in: Neuron, Vol. 21, Issue 5, pp. 1079-92, (1999) (PubMed).

Chen, Chédotal, He, Goodman, Tessier-Lavigne: "Neuropilin-2, a novel member of the neuropilin family, is a high affinity receptor for the semaphorins Sema E and Sema IV but not Sema III." in: Neuron, Vol. 19, Issue 3, pp. 547-59, (1997) (PubMed).

Kolodkin, Levengood, Rowe, Tai, Giger, Ginty: "Neuropilin is a semaphorin III receptor." in: Cell, Vol. 90, Issue 4, pp. 753-62, (1997) (PubMed).

Target Details for GUCA1B

Target: GUCA1B (Guanylate Cyclase Activator 1B (Retina) (GUCA1B))

Alternative Name: GCAP2 / GUCA1B (GUCA1B Products)

Background: Name/Gene ID: GUCA1B

Synonyms: GUCA1B, GCAP2, Guanylate cyclase activator 1B, RP48, GCAP 2, GUCA2

Gene ID: 2979

NCBI Accession: NP_002089

UniProt: Q9UMX6

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction