ELMO1 antibody (AA 372-421)

Catalog No. ABIN6746630

Product Details anti-ELMO1 Antibody

Target: ELMO1 (Engulfment and Cell Motility 1 (ELMO1))

Binding Specificity: AA 372-421

Reactivity: Human, Mouse, Rat, Cow, Zebrafish (Danio rerio), Dog, Horse, Rabbit, Guinea Pig, Bat, Chicken, Hamster, Monkey, Pig, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ELMO1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human ELMO1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Horse, Pig, Chicken, Xenopus, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa372-421 of human ELMO1 (Q92556, NP_055615). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus, Salmon, Stickleback, Zebrafish (100%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-ELMO1 antibody (ABIN6746630)

Moro, Sirrenberg, Schneider, Neupert, Brunner: "The TIM17.23 preprotein translocase of mitochondria: composition and function in protein transport into the matrix." in: The EMBO journal, Vol. 18, Issue 13, pp. 3667-75, (1999) (PubMed).

Rassow, Dekker, van Wilpe, Meijer, Soll: "The preprotein translocase of the mitochondrial inner membrane: function and evolution." in: Journal of molecular biology, Vol. 286, Issue 1, pp. 105-20, (1999) (PubMed).

Ryan, Leung, Jensen: "Characterization of the mitochondrial inner membrane translocase complex: the Tim23p hydrophobic domain interacts with Tim17p but not with other Tim23p molecules." in: Molecular and cellular biology, Vol. 18, Issue 1, pp. 178-87, (1998) (PubMed).

Target Details for ELMO1

Target: ELMO1 (Engulfment and Cell Motility 1 (ELMO1))

Alternative Name: ELMO1 / ELMO 1 (ELMO1 Products)

Synonyms: MGC68475 antibody, ELMO1 antibody, CED-12 antibody, CED12 antibody, ELMO-1 antibody, 6330578D22Rik antibody, C230095H21Rik antibody, ELMO1s antibody, im:7151147 antibody, wu:fq24e08 antibody, zgc:85612 antibody, engulfment and cell motility 1 antibody, engulfment and cell motility 1 S homeolog antibody, engulfment and cell motility 1 (ced-12 homolog, C. elegans) antibody, ELMO1 antibody, elmo1.S antibody, elmo1 antibody, Elmo1 antibody

Background: Name/Gene ID: ELMO1

Synonyms: ELMO1, Ced-12 homolog 1, ELMO-1, Engulfment and cell motility 1, KIAA0281, Protein ced-12 homolog, CED-12, CED12

Gene ID: 9844

NCBI Accession: NP_055615

UniProt: Q92556