COCH antibody (AA 141-190)

Catalog No. ABIN6746617

Product Details anti-COCH Antibody

Target: COCH (Cochlin (COCH))

Binding Specificity: AA 141-190

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Rabbit, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This COCH antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human COCH

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Guinea pig (100%) Horse (92%) Chicken (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa141-190 of human COCH (O43405, NP_001128530). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Dog, Bovine, Rabbit, Guinea pig (100%), Elephant, Panda, Horse (92%), Bat, Opossum, Chicken, Platypus, Lizard, Xenopus (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 0.25 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-COCH antibody (ABIN6746617)

Anfosso, Bardin, Francès, Vivier, Camoin-Jau, Sampol, Dignat-George: "Activation of human endothelial cells via S-endo-1 antigen (CD146) stimulates the tyrosine phosphorylation of focal adhesion kinase p125(FAK)." in: The Journal of biological chemistry, Vol. 273, Issue 41, pp. 26852-6, (1998) (PubMed).

Jean, Gershenwald, Huang, Luca, Hudson, Tainsky, Bar-Eli: "Loss of AP-2 results in up-regulation of MCAM/MUC18 and an increase in tumor growth and metastasis of human melanoma cells." in: The Journal of biological chemistry, Vol. 273, Issue 26, pp. 16501-8, (1998) (PubMed).

Xie, Luca, Huang, Gutman, Reich, Johnson, Bar-Eli: "Expression of MCAM/MUC18 by human melanoma cells leads to increased tumor growth and metastasis." in: Cancer research, Vol. 57, Issue 11, pp. 2295-303, (1997) (PubMed).

Lehmann, Riethmüller, Johnson: "MUC18, a marker of tumor progression in human melanoma, shows sequence similarity to the neural cell adhesion molecules of the immunoglobulin superfamily." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 86, Issue 24, pp. 9891-5, (1990) (PubMed).

Target Details for COCH

Target: COCH (Cochlin (COCH))

Alternative Name: Cochlin / COCH (COCH Products)

Synonyms: AW122937 antibody, Coch-5B2 antibody, D12H14S564E antibody, COCH-5B2 antibody, COCH5B2 antibody, DFNA9 antibody, cochlin antibody, Coch antibody, COCH antibody

Background: Name/Gene ID: COCH

Synonyms: COCH, COCH5B2, COCH-5B2, Cochlin, DFNA9, DFNA31

Gene ID: 1690

NCBI Accession: NP_001128530

UniProt: O43405

Pathways: Sensory Perception of Sound