RAD21 antibody (AA 373-422)

Catalog No. ABIN6746464

Product Details anti-RAD21 Antibody

Target: RAD21 (RAD21 Homolog (RAD21))

Binding Specificity: AA 373-422

Reactivity: Human, Mouse, Rat, Cow, Dog, Horse, Rabbit, Guinea Pig, Monkey, Chicken, Bat, Pig, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAD21 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Rat RAD21

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Horse, Guinea pig, Chicken, Xenopus (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa373-422 of rat Rad21 (Q4KLH7, NP_001020872). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Platypus, Lizard, Xenopus (100%), Salmon, Medaka, Zebrafish (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-RAD21 antibody (ABIN6746464)

Jia, Patwari, Srinivasula, Newland, Fernandes-Alnemri, Alnemri, Kelsey: "Bax translocation is crucial for the sensitivity of leukaemic cells to etoposide-induced apoptosis." in: Oncogene, Vol. 20, Issue 35, pp. 4817-26, (2001) (PubMed).

Hatai, Matsuzawa, Inoshita, Mochida, Kuroda, Sakamaki, Kuida, Yonehara, Ichijo, Takeda: "Execution of apoptosis signal-regulating kinase 1 (ASK1)-induced apoptosis by the mitochondria-dependent caspase activation." in: The Journal of biological chemistry, Vol. 275, Issue 34, pp. 26576-81, (2000) (PubMed).

Target Details for RAD21

Target: RAD21 (RAD21 Homolog (RAD21))

Alternative Name: RAD21 (RAD21 Products)

Synonyms: rad21 antibody, wu:fb55d02 antibody, wu:fb93f04 antibody, CDLS4 antibody, HR21 antibody, HRAD21 antibody, MCD1 antibody, NXP1 antibody, SCC1 antibody, hHR21 antibody, mKIAA0078 antibody, hr21 antibody, mcd1 antibody, nxp-1 antibody, nxp1 antibody, scc1 antibody, xrad21 antibody, RAD21 cohesin complex component antibody, RAD21 cohesin complex component a antibody, RAD21 cohesin complex component L homeolog antibody, RAD21 cohesin complex component S homeolog antibody, R-spondin 2 antibody, RAD21 antibody, rad21a antibody, rad21.L antibody, Rad21 antibody, rad21.S antibody, RSPO2 antibody

Background: Name/Gene ID: RAD21

Synonyms: RAD21, CDLS4, HR21, KIAA0078, HHR21, NXP-1, NXP1, RAD21 (S. pombe) homolog, MCD1, Nuclear matrix protein 1, RAD21 homolog (S. pombe), HRAD21, SCC1, SCC1 homolog

Gene ID: 5885

NCBI Accession: NP_001020872

UniProt: O60216

Pathways: Positive Regulation of Endopeptidase Activity