PFKFB2 antibody (AA 336-385)

Catalog No. ABIN6746455

Product Details anti-PFKFB2 Antibody

Target: PFKFB2 (6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 2 (PFKFB2))

Binding Specificity: AA 336-385

Reactivity: Human, Mouse, Rat, Monkey, Horse, Dog, Zebrafish (Danio rerio), Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PFKFB2 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Mouse PFKFB2

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Horse, Chicken (100%) Bovine, Rabbit, Pig (92%) Zebrafish (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa336-385 of mouse Pfkfb2 (Q6GTL7, NP_032851). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Horse, Opossum, Turkey, Zebra finch, Chicken, Platypus (100%), Bovine, Bat, Rabbit, Pig, Guinea pig, Xenopus (92%), Zebrafish (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-PFKFB2 antibody (ABIN6746455)

Jaiswal, Marlow, Gupta, Narayan: "Beta-catenin-mediated transactivation and cell-cell adhesion pathways are important in curcumin (diferuylmethane)-induced growth arrest and apoptosis in colon cancer cells." in: Oncogene, Vol. 21, Issue 55, pp. 8414-27, (2002) (PubMed).

Target Details for PFKFB2

Target: PFKFB2 (6-phosphofructo-2-Kinase/fructose-2,6-Biphosphatase 2 (PFKFB2))

Alternative Name: PFKFB2 (PFKFB2 Products)

Synonyms: pfkfb2 antibody, zgc:56282 antibody, PFK-2/FBPase-2 antibody, 4930568D07Rik antibody, RH2K antibody, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2a antibody, 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 2 antibody, pfkfb2a antibody, PFKFB2 antibody, pfkfb2 antibody, Pfkfb2 antibody

Background: Name/Gene ID: PFKFB2

Synonyms: PFKFB2, 6PF-2-K/Fru-2,6-P2ase 2, PFK-2/FBPase-2, PFK/FBPase 2, PFKFB, cardiac

Gene ID: 5208

NCBI Accession: NP_032851

UniProt: O60825

Pathways: PI3K-Akt Signaling, Positive Regulation of Peptide Hormone Secretion, Regulation of Carbohydrate Metabolic Process