SEC31A antibody (AA 110-159)

Catalog No. ABIN6746400

Product Details anti-SEC31A Antibody

Target: SEC31A (SEC31 Homolog A (SEC31A))

Binding Specificity: AA 110-159

Reactivity: Human, Mouse, Rat, Cow, Dog, Guinea Pig, Horse, Pig, Rabbit, Bat, Chicken, Monkey, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SEC31A antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Rat SEC31A

Predicted Reactivity: Percent identity by BLAST analysis: Human, Rat, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Chicken (100%) Zebrafish (84%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa110-159 of rat Sec31a (Q9Z2Q1, NP_148981). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Chicken, Platypus, Lizard, Xenopus, Stickleback (100%), Seabass, Zebrafish (90%), Sea squirt (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-SEC31A antibody (ABIN6746400)

Aoudjit, Vuori: "Integrin signaling inhibits paclitaxel-induced apoptosis in breast cancer cells." in: Oncogene, Vol. 20, Issue 36, pp. 4995-5004, (2001) (PubMed).

Rytömaa, Lehmann, Downward: "Matrix detachment induces caspase-dependent cytochrome c release from mitochondria: inhibition by PKB/Akt but not Raf signalling." in: Oncogene, Vol. 19, Issue 39, pp. 4461-8, (2000) (PubMed).

Target Details for SEC31A

Target: SEC31A (SEC31 Homolog A (SEC31A))

Alternative Name: SEC31A / HSPC275 (SEC31A Products)

Synonyms: ABP125 antibody, ABP130 antibody, HSPC334 antibody, SEC31L1 antibody, Sec31l1 antibody, VAP1 antibody, 1810024J13Rik antibody, AU042160 antibody, HSPC275 antibody, cb354 antibody, sec31p antibody, zgc:63547 antibody, SEC31 homolog A, COPII coat complex component antibody, Sec31 homolog A (S. cerevisiae) antibody, SEC31A antibody, Sec31a antibody, sec31a antibody

Background: Name/Gene ID: SEC31A

Synonyms: SEC31A, ABP130, ABP125, HSPC334, HSPC275, SEC31-like 1 (S. cerevisiae), SEC31-like protein 1, SEC31-related protein A, SEC31L1, Web1-like protein, Yeast Sec31p homolog, KIAA0905

Gene ID: 22872

NCBI Accession: NP_148981

UniProt: O94979

Pathways: ER-Nucleus Signaling