GTF2A1L antibody (AA 351-400)

Catalog No. ABIN6746377

Product Details anti-GTF2A1L Antibody

Target: GTF2A1L (General Transcription Factor IIA, 1-Like (GTF2A1L))

Binding Specificity: AA 351-400

Reactivity: Human, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GTF2A1L antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human GTF2A1L / ALF

Predicted Reactivity: Percent identity by BLAST analysis: Human (100%) Dog (92%) Rat (90%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa351-400 of human GTF2A1L. Percent identity by BLAST analysis: Human, Chimpanzee (100%), Gibbon, Horse, Opossum (92%), Mouse, Rat (90%), Gorilla, Monkey, Panda (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-GTF2A1L antibody (ABIN6746377)

Arsham, Plas, Thompson, Simon: "Phosphatidylinositol 3-kinase/Akt signaling is neither required for hypoxic stabilization of HIF-1 alpha nor sufficient for HIF-1-dependent target gene transcription." in: The Journal of biological chemistry, Vol. 277, Issue 17, pp. 15162-70, (2002) (PubMed).

Wiesener, Münchenhagen, Berger, Morgan, Roigas, Schwiertz, Jürgensen, Gruber, Maxwell, Löning, Frei, Maher, Gröne, Eckardt: "Constitutive activation of hypoxia-inducible genes related to overexpression of hypoxia-inducible factor-1alpha in clear cell renal carcinomas." in: Cancer research, Vol. 61, Issue 13, pp. 5215-22, (2001) (PubMed).

Target Details for GTF2A1L

Target: GTF2A1L (General Transcription Factor IIA, 1-Like (GTF2A1L))

Alternative Name: GTF2A1L / ALF (GTF2A1L Products)

Synonyms: ALF antibody, 1700011N16Rik antibody, Alf antibody, Gtf2a1lf antibody, general transcription factor IIA subunit 1 like antibody, general transcription factor IIA, 1-like antibody, general transcription factor 2A subunit 1 like antibody, GTF2A1L antibody, Gtf2a1l antibody

Background: Name/Gene ID: GTF2A1L

Synonyms: GTF2A1L, ALF, GTF2A1-like factor, GTF2A1LF, TFIIA-alpha/beta-like factor

Gene ID: 11036

UniProt: Q9UNN4