NUP37 antibody (AA 53-102)

Catalog No. ABIN6746266

Product Details anti-NUP37 Antibody

Target: NUP37 (Nucleoporin 37kDa (NUP37))

Binding Specificity: AA 53-102

Reactivity: Human, Mouse, Cow, Guinea Pig, Horse, Rat, Hamster, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NUP37 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Mouse NUP37

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Horse (100%) Dog, Chicken (92%) Pig (85%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa53-102 of mouse Nup37 (Q9CWU9, NP_081467). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Bovine, Horse, Guinea pig, Salmon (100%), Panda, Dog, Opossum, Turkey, Zebra finch, Chicken, Platypus (92%), Elephant, Pig (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-NUP37 antibody (ABIN6746266)

Nakatani, Thompson, Barthel, Sakaue, Liu, Weigel, Roth: "Up-regulation of Akt3 in estrogen receptor-deficient breast cancers and androgen-independent prostate cancer lines." in: The Journal of biological chemistry, Vol. 274, Issue 31, pp. 21528-32, (1999) (PubMed).

Frech, Andjelkovic, Ingley, Reddy, Falck, Hemmings: "High affinity binding of inositol phosphates and phosphoinositides to the pleckstrin homology domain of RAC/protein kinase B and their influence on kinase activity." in: The Journal of biological chemistry, Vol. 272, Issue 13, pp. 8474-81, (1997) (PubMed).

Cross, Alessi, Cohen, Andjelkovich, Hemmings: "Inhibition of glycogen synthase kinase-3 by insulin mediated by protein kinase B." in: Nature, Vol. 378, Issue 6559, pp. 785-9, (1996) (PubMed).

Jones, Jakubowicz, Pitossi, Maurer, Hemmings: "Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 10, pp. 4171-5, (1991) (PubMed).

Target Details for NUP37

Target: NUP37 (Nucleoporin 37kDa (NUP37))

Alternative Name: NUP37 (NUP37 Products)

Synonyms: MGC83926 antibody, 2410003L22Rik antibody, 2810039M17Rik antibody, p37 antibody, nucleoporin 37kDa L homeolog antibody, nucleoporin 37 antibody, nucleoporin 37kDa antibody, nup37.L antibody, NUP37 antibody, nup37 antibody, Nup37 antibody

Background: Name/Gene ID: NUP37

Synonyms: NUP37, Nucleoporin 37  kDa, p37, Nucleoporin Nup37

Gene ID: 79023

NCBI Accession: NP_081467

UniProt: Q8NFH4