B3GALNT1 antibody (AA 204-253)

Catalog No. ABIN6746248

Product Details anti-B3GALNT1 Antibody

Target: B3GALNT1 (beta-1,3-N-Acetylgalactosaminyltransferase 1 (B3GALNT1))

Binding Specificity: AA 204-253

Reactivity: Human, Mouse, Rat, Horse, Rabbit, Cow, Dog, Guinea Pig, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This B3GALNT1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Rat B3GALNT1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Dog, Bovine, Horse (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa204-253 of rat B3galnt1 (Q6AY39, NP_001013176). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Rabbit, Horse, Pig, Guinea pig, Zebra finch (100%), Platypus (92%), Opossum (85%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-B3GALNT1 antibody (ABIN6746248)

Jayaraman, Murthy, Zhu, Curran, Xanthoudakis, Prives: "Identification of redox/repair protein Ref-1 as a potent activator of p53." in: Genes & development, Vol. 11, Issue 5, pp. 558-70, (1997) (PubMed).

Xanthoudakis, Miao, Curran: "The redox and DNA-repair activities of Ref-1 are encoded by nonoverlapping domains." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 91, Issue 1, pp. 23-7, (1994) (PubMed).

Xanthoudakis, Miao, Wang, Pan, Curran: "Redox activation of Fos-Jun DNA binding activity is mediated by a DNA repair enzyme." in: The EMBO journal, Vol. 11, Issue 9, pp. 3323-35, (1992) (PubMed).

Target Details for B3GALNT1

Target: B3GALNT1 (beta-1,3-N-Acetylgalactosaminyltransferase 1 (B3GALNT1))

Alternative Name: B3GALNT1 (B3GALNT1 Products)

Synonyms: B3GALT3 antibody, B3galt3 antibody, b3GT3 antibody, GLCT3 antibody, GLOB antibody, Gb4Cer antibody, P antibody, P1 antibody, beta3Gal-T3 antibody, galT3 antibody, beta-1,3-N-acetylgalactosaminyltransferase 1 (globoside blood group) antibody, beta-1,3-N-acetylgalactosaminyltransferase 1 antibody, UDP-GalNAc:betaGlcNAc beta 1,3-galactosaminyltransferase, polypeptide 1 antibody, B3GALNT1 antibody, B3galnt1 antibody

Background: Name/Gene ID: B3GALNT1

Synonyms: B3GALNT1, Beta3Gal-T3, Beta3GalT3, B3Gal-T3, Beta-1,3-GalTase 3, B3GALT3, Globoside synthase, GLCT3, GLOB, GalT3, Gb4Cer, P antigen synthase, p1, Beta-1,3-GalNAc-T1, Beta-3-Gx-T3, Brainiac1, P blood group globoside

Gene ID: 8706

NCBI Accession: NP_001013176

UniProt: O75752