SNRPN antibody (AA 46-95)

Catalog No. ABIN6746188

Product Details anti-SNRPN Antibody

Target: SNRPN (Small Nuclear Ribonucleoprotein Polypeptide N (SNRPN))

Binding Specificity: AA 46-95

Reactivity: Human, Mouse, Rat, Dog, Cow, Guinea Pig, Rabbit, Horse, Monkey, Pig

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SNRPN antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Human SNRPN

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Rabbit, Guinea pig (100%) Xenopus (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa46-95 of human SNRPN (P63162, NP_003088). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig (100%), Turkey, Zebra finch, Chicken, Xenopus, Salmon, Stickleback, Zebrafish (92%), Panda, Bat (85%), Drosophila (83%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-SNRPN antibody (ABIN6746188)

Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." in: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, (2002) (PubMed).

Buschmann, Lerner, Lee, Ronai: "The Mdm-2 amino terminus is required for Mdm2 binding and SUMO-1 conjugation by the E2 SUMO-1 conjugating enzyme Ubc9." in: The Journal of biological chemistry, Vol. 276, Issue 44, pp. 40389-95, (2001) (PubMed).

Kovalenko, Plug, Haaf, Gonda, Ashley, Ward, Radding, Golub: "Mammalian ubiquitin-conjugating enzyme Ubc9 interacts with Rad51 recombination protein and localizes in synaptonemal complexes." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 7, pp. 2958-63, (1996) (PubMed).

Target Details for SNRPN

Target: SNRPN (Small Nuclear Ribonucleoprotein Polypeptide N (SNRPN))

Alternative Name: SNRPN (SNRPN Products)

Synonyms: SNRB' antibody, SNRPB' antibody, Snrpn antibody, zgc:77315 antibody, HCERN3 antibody, PWCR antibody, RT-LI antibody, SM-D antibody, SMN antibody, SNRNP-N antibody, SNURF-SNRPN antibody, sm-N antibody, 2410045I01Rik antibody, Peg4 antibody, Pwcr1 antibody, FE 294 antibody, FE 294 psi antibody, FE294 antibody, FE294 psi antibody, SNURF antibody, SNRPN antibody, QflA-11237 antibody, QtrA-10022 antibody, Sm-N antibody, SmN antibody, snRNP-N antibody, small nuclear ribonucleoprotein polypeptide-like antibody, small nuclear ribonucleoprotein polypeptides B and B1 antibody, small nuclear ribonucleoprotein polypeptide N antibody, small nuclear ribonucleoprotein N antibody, small nuclear ribonucleoparticle-associated protein (snRNP) mRNA, clone Sm51 antibody, SNRPN upstream reading frame antibody, Small nuclear ribonucleoprotein-associated protein N antibody, SNRPL antibody, snrpb antibody, SNRPN antibody, Snrpn antibody, snrpn antibody, SNURF antibody

Background: Name/Gene ID: SNRPN

Synonyms: SNRPN, HCERN3, RT-LI, Sm protein D, Sm-N, Sm protein N, SMN, SNURF-SNRPN, PWCR, SM-D, SNRNP-N

Gene ID: 6638

NCBI Accession: NP_003088