FMR1 antibody (AA 421-470)

Catalog No. ABIN6746187

Product Details anti-FMR1 Antibody

Target: FMR1 (Fragile X Mental Retardation 1 (FMR1))

Binding Specificity: AA 421-470

Reactivity: Human, Mouse, Rat, Dog, Monkey, Rabbit, Pig, Cow, Bat, Guinea Pig, Chicken

Host: Rabbit

Clonality: Polyclonal

Conjugate: This FMR1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Rat FMR1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Rabbit, Pig, Guinea pig, Chicken (100%) Xenopus (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa421-470 of rat Fmr1 (Q80WE1, NP_434691). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Bat, Rabbit, Pig, Guinea pig, Turkey, Chicken (100%), Platypus, Xenopus (92%), Zebra finch (91%), Opossum (84%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-FMR1 antibody (ABIN6746187)

Devarajan, De Leon, Talasazan, Schoenfeld, Davidowitz, Burk: "The von Hippel-Lindau gene product inhibits renal cell apoptosis via Bcl-2-dependent pathways." in: The Journal of biological chemistry, Vol. 276, Issue 44, pp. 40599-605, (2001) (PubMed).

Anderson, Teutsch, Dong, Wortis: "An essential role for Bruton's [corrected] tyrosine kinase in the regulation of B-cell apoptosis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 20, pp. 10966-71, (1996) (PubMed).

Target Details for FMR1

Target: FMR1 (Fragile X Mental Retardation 1 (FMR1))

Alternative Name: FMR1 / FMRP (FMR1 Products)

Background: Name/Gene ID: FMR1

Synonyms: FMR1, FMRP, POF, Protein FMR-1, Premature ovarian failure 1, Fragile X mental retardation 1, FRAXA, POF1

Gene ID: 2332

NCBI Accession: NP_434691

UniProt: Q06787

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development