AUH antibody (AA 38-87)

Catalog No. ABIN6746186

Product Details anti-AUH Antibody

Target: AUH (AU RNA Binding Protein/enoyl-CoA Hydratase (AUH))

Binding Specificity: AA 38-87

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AUH antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Mouse AUH

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat (100%) Bovine, Rabbit (92%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide located between aa38-87 of mouse Auh (Q9JLZ3, NP_057918). Percent identity by BLAST analysis: Human, Gorilla, Monkey, Mouse, Rat (100%), Gibbon, Galago, Marmoset, Panda, Bovine, Rabbit (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (0.2 - 1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Mouse

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-AUH antibody (ABIN6746186)

Devarajan, De Leon, Talasazan, Schoenfeld, Davidowitz, Burk: "The von Hippel-Lindau gene product inhibits renal cell apoptosis via Bcl-2-dependent pathways." in: The Journal of biological chemistry, Vol. 276, Issue 44, pp. 40599-605, (2001) (PubMed).

Anderson, Teutsch, Dong, Wortis: "An essential role for Bruton's [corrected] tyrosine kinase in the regulation of B-cell apoptosis." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, Issue 20, pp. 10966-71, (1996) (PubMed).

Target Details for AUH

Target: AUH (AU RNA Binding Protein/enoyl-CoA Hydratase (AUH))

Alternative Name: AUH (AUH Products)

Synonyms: C77140 antibody, W91705 antibody, wu:fb81b10 antibody, zgc:101057 antibody, AU RNA binding methylglutaconyl-CoA hydratase antibody, AU RNA binding protein/enoyl-coenzyme A hydratase antibody, AU RNA binding protein/enoyl-CoA hydratase antibody, AU RNA binding protein/enoyl-CoA hydratase S homeolog antibody, AUH antibody, Auh antibody, auh antibody, auh.S antibody

Background: Name/Gene ID: AUH

Synonyms: AUH

Gene ID: 549

NCBI Accession: NP_057918

UniProt: Q13825