TCEA1 antibody (C-Term)

Catalog No. ABIN6746165

Product Details anti-TCEA1 Antibody

Target: TCEA1 (Transcription Elongation Factor A Protein 1 (TCEA1))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Dog, Guinea Pig, Horse, Rabbit, Cow, Zebrafish (Danio rerio), Monkey, Pig, Bat, Chicken, Xenopus laevis, Drosophila melanogaster, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TCEA1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: Rat TCEA1

Predicted Reactivity: Percent identity by BLAST analysis: Human, Mouse, Rat, Bovine, Guinea pig, Chicken, Xenopus, Zebrafish (100%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide from C-Terminus of rat Tcea1 (Q4KLL0, NP_001020906). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Baboon, Monkey, Marmoset, Mouse, Rat, Shrew, Hamster, Elephant, Panda, Dog, Bovine, Bat, Rabbit, Horse, Pig, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Armadillo, Platypus, Xenopus, Frog, Trout, Catfish, Salmon, Sablefish, Stickleback, Pike, Zebrafish, Drosophila, Rice, Grape (100%), Goat, Sea squirt, Beetle (92%).

Type of Immunogen: Synthetic peptide

Application Details

Application Notes: Approved: WB (1 μg/mL)

Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Comment:

Target Species of Antibody: Rat

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Distilled water

Concentration: Lot specific

Buffer: Lyophilized from PBS with 2 % sucrose

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.

References for anti-TCEA1 antibody (ABIN6746165)

Stenmark, Vitale, Ullrich, Zerial: "Rabaptin-5 is a direct effector of the small GTPase Rab5 in endocytic membrane fusion." in: Cell, Vol. 83, Issue 3, pp. 423-32, (1996) (PubMed).

Ullrich, Horiuchi, Bucci, Zerial: "Membrane association of Rab5 mediated by GDP-dissociation inhibitor and accompanied by GDP/GTP exchange." in: Nature, Vol. 368, Issue 6467, pp. 157-60, (1994) (PubMed).

Li, Stahl: "Structure-function relationship of the small GTPase rab5." in: The Journal of biological chemistry, Vol. 268, Issue 32, pp. 24475-80, (1993) (PubMed).

Sanford, Pan, Wessling-Resnick: "Prenylation of Rab5 is dependent on guanine nucleotide binding." in: The Journal of biological chemistry, Vol. 268, Issue 32, pp. 23773-6, (1993) (PubMed).

Target Details for TCEA1

Target: TCEA1 (Transcription Elongation Factor A Protein 1 (TCEA1))

Alternative Name: TCEA1 / TFIIS (TCEA1 Products)

Synonyms: gtf2s antibody, sii antibody, tcea antibody, tf2s antibody, TCEA1 antibody, S-II antibody, GTF2S antibody, SII antibody, TCEA antibody, TF2S antibody, TFIIS antibody, fj48e12 antibody, wu:fj48e12 antibody, zgc:63975 antibody, transcription elongation factor A (SII), 1 antibody, transcription elongation factor A1 antibody, transcription elongation factor A (SII) 1 antibody, transcription elongation factor A (SII), 1 L homeolog antibody, tcea1 antibody, TCEA1 antibody, Tcea1 antibody, tcea1.L antibody

Background: Name/Gene ID: TCEA1

Synonyms: TCEA1, GTF2S, SII, TCEA, TFIIS

Gene ID: 6917

NCBI Accession: NP_001020906

UniProt: P23193